Table 4 Dilution integrity (n = 5)
Compound | Concentration before dilution (ng/mL) | Dilution factors | Concentration after dilution (ng/mL) | Determined concentration (ng/mL) | % Bias | % CV |
|---|---|---|---|---|---|---|
EtG | 250 | 10 | 25 | 24.4 | − 2.5 | 8.2 |
5 | 50 | 51.1 | 2.2 | 5.5 | ||
800 | 40 | 20 | 20.6 | 2.8 | 6.8 | |
20 | 40 | 40.0 | 0.1 | 6.0 | ||
10 | 80 | 80.6 | 0.7 | 2.0 | ||
NTX | 20 | 10 | 2 | 2.0 | − 1.4 | 4.9 |
5 | 4 | 4.0 | 0.5 | 8.0 | ||
80 | 40 | 2 | 1.9 | − 5.4 | 3.2 | |
20 | 4 | 3.7 | − 7.1 | 5.3 | ||
10 | 8 | 7.3 | − 8.3 | 5.2 | ||
6βNTX | 250 | 10 | 25 | 25.2 | 0.9 | 8.3 |
5 | 50 | 50.9 | 1.8 | 8.1 | ||
800 | 40 | 20 | 19.0 | − 5.2 | 7.9 | |
20 | 40 | 39.6 | − 1.1 | 4.5 | ||
10 | 80 | 76.8 | − 4.0 | 7.3 | ||
CDP | 250 | 10 | 25 | 24.1 | − 3.6 | 9.4 |
5 | 50 | 47.3 | − 5.5 | 6.7 | ||
800 | 40 | 20 | 19.6 | − 2.2 | 4.7 | |
20 | 40 | 39.8 | − 0.5 | 10.0 | ||
10 | 80 | 81.8 | 2.2 | 4.8 | ||
norCDP | 250 | 10 | 25 | 23.9 | − 4.5 | 2.8 |
5 | 50 | 50.2 | 0.4 | 3.4 | ||
800 | 40 | 20 | 19.0 | − 5.2 | 6.2 | |
20 | 40 | 38.6 | − 3.4 | 8.7 | ||
10 | 80 | 78.0 | − 2.6 | 3.4 |