Table 3 Intra- and inter-day accuracy and precision, recovery (RE), and matrix effect (ME) used to validate the method using quality control (QC) samples
Compound | QC sample (ng/mL) | Intra-day (n = 5) | Inter-day (n = 15) | RE (n = 6) % | ME (n = 6) % | ||
|---|---|---|---|---|---|---|---|
% Bias | % CV | % Bias | % CV | ||||
EtG | 5 | − 4.7 | 3.8 | − 8.4 | 8.1 | 89.7 | 99.6 |
15 | − 1.4 | 6.7 | − 0.2 | 7.1 | 90.6 | 102.0 | |
250 | − 1.3 | 1.8 | 2.0 | 6.3 | 97.7 | 96.0 | |
800 | − 0.3 | 0.9 | 3.4 | 6.8 | 98.0 | 98.3 | |
NTX | 1 | 7.5 | 6.5 | 7.8 | 6.8 | 95.9 | 52.2 |
3 | − 2.3 | 6.7 | − 5.3 | 5.2 | 86.9 | 49.0 | |
20 | − 2.2 | 6.3 | − 3.8 | 6.8 | 103.0 | 50.3 | |
80 | 11.4 | 1.1 | 8.3 | 5.3 | 93.1 | 52.0 | |
6βNTX | 5 | − 7.8 | 9.5 | − 2.7 | 15.2 | 95.0 | 89.2 |
15 | − 7.3 | 7.2 | − 1.0 | 10.1 | 88.6 | 88.1 | |
250 | − 4.3 | 5.3 | − 0.7 | 7.6 | 103.9 | 77.6 | |
800 | 0.1 | 4.3 | − 4.4 | 6.7 | 104.2 | 71.7 | |
CDP | 5 | 11.2 | 5.2 | 2.3 | 10.0 | 89.7 | 48.9 |
15 | 5.9 | 6.6 | 2.7 | 7.8 | 93.8 | 46.3 | |
250 | − 0.4 | 7.0 | 1.3 | 5.9 | 96.6 | 47.0 | |
800 | 3.9 | 6.6 | 2.2 | 7.2 | 91.2 | 49.4 | |
norCDP | 5 | − 5.0 | 5.3 | − 0.5 | 6.3 | 95.3 | 45.3 |
15 | 1.3 | 6.1 | − 0.3 | 4.6 | 90.1 | 42.9 | |
250 | 8.8 | 3.0 | 2.8 | 5.6 | 103.0 | 42.1 | |
800 | 1.0 | 2.1 | 0.8 | 3.7 | 100.6 | 43.0 | |