Fig. 5
The chemical shift perturbations of a–c holo, butyryl, and isobutyryl ACP in 1H–15N HSQC spectra from the apo forms of C. acnes. CSPs (Δδ) are calculated using the equation Δδ = (0.5(Δδ(1H)2 + (αΔδ(15N)2)))1/2 (α = 0.2 for most residues, and α = 0.14 for glycine). Structural representations of residues showing large CSPs in d holo-CaACP, e butyryl CaACP, and f isobutyryl CaACP (CSP > 0.2 red, > 0.1 yellow, > 0.05 green)