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Journal of Analytical Science and Technology
Fig. 1 | Journal of Analytical Science and Technology

Fig. 1

From: Microscopic studies on severing properties of actin-binding protein: its potential use in therapeutic treatment of actin-rich inclusions

Fig. 1

Electron microscopic analysis of actin-severing and bundling activities of gelsolin and ABP34 in the presence of Ca2+. Negative staining (NS) electron micrographs taken from F-actin alone (a, b); mixture of F-actin and gelsolin (c, d); mixture of F-actin and ABP34 (e, f). All the samples were mixed in low ionic strength buffer (at a molar ratio of 1:1; final concentration 3 μM). In the presence of Ca2+, actin-binding proteins typically exhibited their native activity. NS-electron micrographs were taken at both low 4200 (a, c and e; left panels) and high 34,000 (b, d and f; right panels) nominal magnifications. Scale bars represent 2 μm (a, c and e) and 200 nm (b, d and f) in each field

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