Development and application of a chemical profiling method for the assessment of the quality and consistency of the Pelargonium sidoides extract

Journal of Analytical Science and Technology

Table 2 Linearity, precision, and accuracy test for method validation

Peak	a	b	c	d	e	f	g
Linearity, LOD, LOQ
PMM (R²)	0.9994	0.9991	0.9975	0.9891	0.9981	0.9983	0.9952
Standard (R²)	0.9999	0.9997	–	–	–	–	0.9996
LOD (µg/ml)	0.1629	0.2507	–	–	–	–	0.2450
LOQ (µg/ml)	0.4937	0.7597	–	–	–	–	0.7425
Repeatability (intra-day), RSD (%)
RT	0.4304	0.3775	0.3304	0.1765	0.0727	0.0670	0.0714
RRT	0.0000	0.0581	0.1445	0.2625	0.3578	0.3653	0.3787
PA	1.427	1.404	3.027	3.681	2.663	3.277	2.646
RPA	0.000	1.919	3.097	2.985	2.536	3.034	3.146
Intermediate precision (inter-day), RSD (%)
RT	0.2904	0.3194	0.2237	0.3616	0.1231	0.1259	0.0422
RRT	0.0000	0.0454	0.0710	0.0736	0.1702	0.1710	0.2591
PA	0.4555	1.324	4.972	3.151	3.169	2.308	0.2796
RPA	0.0000	1.309	4.707	2.814	2.804	1.942	0.6872
Accuracy (recovery %)
80 (mg/10 ml)	100.3	99.45	102.2	99.21	102.3	100.6	102.7
100 (mg/10 ml)	101.7	102.3	100.1	106.8	100.5	102.3	98.85
120 (mg/10 ml)	100.0	99.77	96.84	96.79	98.50	99.23	98.57