Performance comparison between solid phase extraction and magnetic carbon nanotubes facilitated dispersive-micro solid phase extractions (Mag-CNTs/d-µSPE) of a cyanide metabolite in biological samples using GC–MS

Journal of Analytical Science and Technology

Table 1 Extraction parameters for the two Mag-CNTs/d-µSPE methods and the SPE methods

	Mag-CNTs-COOH/d-µSPE (Li et al. 2019a)^a		Mag-CNTs-COOH/d-µSPE		Mag-CNTs-SO₃H/d-µSPE		SPE
Extraction Parameters	Synthetic urine	Bovine blood	Synthetic urine	Bovine blood	Synthetic urine	Bovine blood	Bovine blood
Sample Volume (µL)	100	100	500	500	1000	200	500
Amount of 0.1 M HCl	2% (v/v)^b	2% (v/v)^b	5% (v/v)	5% (v/v)	5% (v/v)	2.5% (v/v)	1 mL
Amount of sorbent (mg)	2	2	5	5	10	5	35
Extraction Time (min)	10	10	20	20	10	20	N/A
Elution Solvent	5% (v/v) NH₄OH in water	5% (v/v) NH₄OH in water	N/A	N/A	DCM/IPA/NH₄OH (78/20/2)	DCM/IPA/NH₄OH (78/20/2)	5% (v/v) NH₄OH in MeOH
Elution volume	150 µL	150 µL	N/A	N/A	1 mL	1 mL	1 mL
Derivatization solvent	30% (v/v) MSTFA in hexane	30% (v/v) MSTFA in hexane	30% (v/v) MSTFA in acetone	30% (v/v) MSTFA in acetone	30% (v/v) MSTFA in hexane	30% (v/v) MSTFA in hexane	30% (v/v) MSTFA in hexane
Derivatization Volume (µL)	150	150	50	50	50	50	50
Derivatization Time (min)	10	10	10	10	10	10	10
Solvent used^c (µL)	152	352	25	1150	1050	1440	6100