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Table. 8 Stability results after storage of methyl esters at different temperatures

From: Standardization and validation of assay of selected omega-3 and omega-6 fatty acids from phospholipid fraction of red cell membrane using gas chromatography with flame ionization detector

 

% peak composition (absolute difference)

Fatty acids

RT

4 – 8o

 

Day 1

Day 3

Day 5

Day 8

Day 1

Day 3

Day 5

Day 8

ALA, C 18:3, (n-3)

0.46

0.44 (0.02)

0.33 (0.13)

0.27 (0.19)

0.43

0.41 (0.02)

0.34 (0.09)

0.26 (0.17)

EPA, C 20:5, (n-3)

0.46

0.35 (0.11)

0.24 (0.22)

0.11 (0.35)

0.26

0.17 (0.08)

0.20 (0.06)

0.18 (0.08)

DHA, C 22:6, (n-3)

1.22

1.10 (0.12)

1.18 (0.04)

1.03 (0.19)

1.12

1.16 (-0.04)

1.10 (0.01)

1.05 (0.07)

LA, C 18:2, (n-6)

5.66

5.49 (0.17)

5.78 (-0.12)

5.80 (-0.14)

5.65

5.64 (0.01)

5.74 (-0.10)

5.81 (-0.17)

GLA, C 18:3, (n-6)

0.41

0.46 (-0.04)

0.35 (0.06)

0.12 (0.29)

0.43

0.47 (-0.04)

0.28 (0.15)

0.23 (0.20)

AA, C 20:4, (n-6)

6.12

6.01 (0.11)

6.10 (0.02)

6.23 (-0.11)

6.06

6.03 (0.02)

6.17 (-0.12)

6.29 (-0.24)

  1. RT room temperature, ALA alpha linolenic acid, EPA eicosapentaenoic acid, DHA docosahexaenoic acid, LA linoleic acid, GLA gamma-linolenic acid, AA arachidonic acid