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Table. 6 Linearity with sample concentration

From: Standardization and validation of assay of selected omega-3 and omega-6 fatty acids from phospholipid fraction of red cell membrane using gas chromatography with flame ionization detector

Fatty acids

Concentration range (μg/ml)

Regression equation Y = ax + b

Correlation coefficient “r

α-Linolenic acid, C18:3 (n-3)

0.2–4

48.528x − 0.4636

0.9971

Eicosapentaenoic acid, C20:5 (n-3)

0.2–4

49.153x − 0.6419

0.9971

Docosahexaenoic acid, C22:6 (n-3)

0.2–4

45.859x − 0.511

0.9965

Linoleic acid, C18:2 (n-6)

0.2–4

49.244x − 0.252

0.9972

γ-linolenic acid, C18:3 (n-6)

0.2–4

48.424x − 0.3464

0.9971

Arachidonic acid, C20:4 (n-6)

0.2–4

47.8x − 0.5545

0.9970