Skip to main content

Table. 2 Agreement of analysis between the two laboratories showing intraclass correlation

From: Standardization and validation of assay of selected omega-3 and omega-6 fatty acids from phospholipid fraction of red cell membrane using gas chromatography with flame ionization detector

Fatty acids

PHFI laboratory (n = 180)

AIIMS laboratory (n = 180)

Mean difference (95% CI)

ICC (95% CI)

Mean ± SD

Median (IQR)

Mean ± SD

Median (IQR)

ALA

C18:3(n-3)

0.4549 ± 0.4651

0.3632 (0.258, 0.552)

0.5032 ± 0.5162

0.3774 (0.288, 0.589)

− 0.0260 (− 0.007, − 0.06)

0.832 (0.775–0.875)

EPA

C20:5(n-3)

0.3107 ± 0.1792

0.2741 (0.203, 0.363)

0.3390 ± 0.1909

0.2981 (0.213, 0.424)

− 0.0284 (− 0.004, − 0.052)

0.752 (0.667–0.816)

DHA

C22:6(n-3)

0.9636 ± 1.0962

0.5027 (0.335, 1.014)

1.0186 ± 1.0658

0.6095 (0.367, 1.101)

− 0.0549 (− 0.016, − 0.093)

0.985 (0.980–0. 989)

LA

C18:2(n-6)

5.8225 ± 2.3579

5.6083 (3.742, 7.501)

5.9080 ± 2.4164

5.6816 (3.827, 7.594)

− 0.0855 (− 0.039, -0.131)

0.996 (0.994–0.997)

GLA

C18:3(n-6)

0.3509 ± 0.1862

0.3106 (0.225, 0.423)

0.4192 ± 0.2421

0.3653 (0.265, 0.505)

− 0.0683 (− 0.037, − 0.099)

0.689 (0.582–0.768)

AA

C20:4(n-6)

4.6899 ± 3.7776

3.2405 (2.249, 5.406)

4.7023 ± 3.8154

3.1651 (2.267, 5.418)

− 0.0124 (0.148, − 0.173)

0.979 (0.971–0.984)

  1. PHFI, Public Health Foundation of India; AIIMS, All India Institute of Medical Sciences; ICC, intraclass correlation coefficient; CI, confidence interval; IQR, interquartile range; ALA, alpha linolenic acid; EPA, eicosapentaenoic acid; DHA, docosahexaenoic acid; LA, linoleic acid; GLA, gamma-linolenic acid; AA, arachidonic acid