Skip to main content

Table. 1 Intra-assay and inter-assay coefficient of variation from RBC pool and commercially available FAME for n-3 and n-6 fatty acids

From: Standardization and validation of assay of selected omega-3 and omega-6 fatty acids from phospholipid fraction of red cell membrane using gas chromatography with flame ionization detector

Fatty acids

Mean concentration, RBC pool (% of total)

Intra-assay CV % (RBC pool)

Inter-assay CV % (RBC pool)

Concentration, FAME

Inter-assay CV % (FAME)

α-Linolenic acid, C18:3 (n-3)

0.22

5.4

11.07

2

6.25

Eicosapentaenoic acid, C20:5 (n-3)

0.60

5.0

13.0

2

5.31

Docosahexaenoic acid, C22:6 (n-3)

1.92

2.0

2.62

2

3.95

Linoleic acid, C18:2 (n-6)

14.07

1.3

2.33

2

3.15

γ-linolenic acid, C18:3 (n-6)

0.99

5.7

6.04

2

6.87

Arachidonic acid, C20:4 (n-6)

5.96

1.19

0.78

2

9.99

  1. CV, coefficient of variation; FAME, fatty acid methyl ester; RBC, red blood cells