Table 3 Analytical results and percent recoveries of RAC and CLEN in pooled animal urine samples
Concentration of RAC (ng mL−1) | Concentration of CLEN (ng mL−1) | |||||
|---|---|---|---|---|---|---|
Sample | Added | Found | Recovery (%) | Added | Found | Recovery (%) |
IT monolithic μ-SPE coupled with HPLC-UV | ||||||
Swine urine | 0 | N.D. | – | 0 | N.D. | – |
31.8 | 32.8±0.02 | 103 | 16.8 | 16.9±0.03 | 101 | |
63.6 | 60.2±0.02 | 95 | 67.2 | 69.1±0.02 | 103 | |
159.0 | 159.7±0.09 | 100 | 168.0 | 167.5±0.01 | 100 | |
Cattle urine | 0 | N.D. | – | 0 | N.D. | – |
31.8 | 30.6±0.03 | 96 | 16.8 | 15.7±0.03 | 93 | |
63.6 | 64.0±0.02 | 101 | 67.2 | 68.3±0.05 | 102 | |
159.0 | 158.3±0.08 | 100 | 168.0 | 165.0±0.16 | 98 | |
IT monolithic μ-SPE coupled with UHPLC-Q ExactiveTM Plus Orbitrap HRMS | ||||||
Swine urine | 0 | N.D. | – | 0 | N.D. | – |
5.3 | 5.2±0.01 | 98 | 3.1 | 3.1±0.05 | 100 | |
63.6 | 61.6±0.01 | 97 | 67.2 | 71.0±0.08 | 106 | |
159.0 | 159.0±0.09 | 100 | 168.0 | 169.8±0.29 | 101 | |
Cattle urine | 0 | N.D. | – | 0 | N.D. | – |
5.3 | 5.2±0.02 | 98 | 3.1 | 3.0±0.03 | 97 | |
63.6 | 60.9±0.01 | 96 | 67.2 | 65.7±0.03 | 98 | |
159.0 | 157.9±0.02 | 99 | 168.0 | 169.7±0.17 | 101 | |