Parameter | Condition | ||
---|---|---|---|
Liquid chromatography conditions | |||
Mobile phase A | Acetonitrile:ammonium formate buffer (pH 6) (95:5). | ||
Mobile phase B | Acetonitrile:ammonium formate buffer (pH 6) (50:50). | ||
Auto-sampler temperature | 25 °C | ||
Column temperature | 35 °C | ||
Flow rate | 0.8 mL/min | ||
Injection volume | 10 μL | ||
Gradient program (time/mobile phase B) | 0/5, 3/10, 6/40, 8/70, 10/70 | ||
Run time | 10 min | ||
Mass spectrometry conditions | |||
Source | ESI | ||
Ionization mode | Positive | ||
Capillary voltage | 3.0 kV | ||
Detection mode | MRM | ||
MRM transitions (m/z) selected | PGI-1 | PGI-2 | PGI-3 |
For qualification | 163.1 > 126.3 | 143.1 > 63.1 | 80.5 > 63.1 |
For quantification | 163.1 > 90.1 | 143.1 > 107.1 | 80.5 > 45.1 |
Sample cone | 15 V | ||
Probe temperature | 150 °C | ||
Heating block temperature | 280 °C | ||
Desolvation (DL) temperature | 300 °C | ||
Nebulization and drying gas | Nitrogen | ||
Nebulization gas flow | 1.0 mL/min | ||
Drying gas flow | 900 L/h | ||
CID gas | Argon |