Fig. 2

a Effect of BR buffer pH on CL emission. Experimental conditions: c(ABEI)=0.037 mmol/L, c(sodium fluorescein)=0.701 mmol/L, c(HRP)=0.098 mg/mL, c(H₂O₂)=0.014 mol/L. b CL kinetic curves of the sodium fluorescein-ABEI–H₂O₂–HRP system at different buffered solution: BR buffer (black curve), PBS buffer (pink curve), Tris-HCl buffer (red curve), HEPES buffer (blue curve). Experimental conditions: c(ABEI)=0.037 mmol/L, c(sodium fluorescein)=0.701 mmol/L, c(HRP)=0.098 mg/mL, c(H₂O₂)=0.014 mol/L. c Effect of sodium fluorescein concentration on CL emission. Experimental conditions: c(BR)=0.018 mmol/L at pH 7.8, c(ABEI)=0.037 mmol/L, c(HRP)=0.098 mg/mL, c(H₂O₂)=0.014 mol/L. d Effect of ABEI concentration on CL emission. Experimental conditions: c(BR)=0.018 mmol/L at pH 7.8, c(sodium fluorescein)=0.701 mmol/L, c(HRP)=0.098 mg/ml, c(H₂O₂)=0.014 mol/L. e Effect of H₂O₂ concentration on CL emission. c(BR)=0.018 mmol/L at pH 7.8, c(ABEI)=0.037 mmol/L, c(Sodium fluorescein)=0.701 mmol/L, c(HRP)=0.098 mg/mL. f Effect of HRP concentration on CL emission. Experimental conditions: c(BR)=0.018 mmol/L at pH 7.8, c(ABEI)=0.037 mmol/L, c(sodium fluorescein)=0.701 mmol/L, c(H₂O₂)=0.014 mol/L