Fig. 2
From: Dimerization of cell-penetrating buforin II enhances antimicrobial properties
Ability of peptides to permeabilize membranes. a Effect of peptides on membrane potential of intact S. aureus cells (OD600 = 0.05). After the fluorescence of DiSC3(5) was stabilized, the peptides (2 μM) were added to S. aureus. b Release of calcein fluorescent probe from anionic PC/PG (1:1) or zwitterionic PC vesicles. Membrane-lytic activity of peptides was defined as the percent leakage from 100 μM lipid after 10 min incubation with peptides (2 μM for PC/PG (1:1) and 20 μM for PC vesicles). Average ± SD for three independent experiments. Statistically significant (p < 0.05) and very significant (p < 0.01) differences are represented by (*) and (**), respectively