Fig. 2

Two metabolic pathway of aniline degradation in Delftia sp. K82. Two metabolic pathways (an intradiol cleavage pathway and an extradiol cleavage pathway) of aniline degradation in Delftia sp. K82 have been identified. Enzymes of each pathway are numbered and are listed in Table 3. The aniline oxygenase complex of Delftia sp. K82 is composed of six enzymes (Nos. 1∼6) and four reaction steps for the conversion of aniline into catechol. The locus_tag and enzyme name of each number are as follows: No.1, KDK82_RS31140, glutamine synthetase; No.2,KDK82_RS31135,glutamine amidotransferase ; No.3,KDK82_RS31130,Large subunit of dioxygenase; No.4,KDK82_RS31125,small subunit of dioxygenase ; No.5,KDK82_RS31120,aniline dioxygenase reductase; No.6,KDK82_RS31110,small ferredoxin-like protein ; No.7,KDK82_RS31105, catechol 2,3-dioxygenase; No.8,KDK82_RS31090, 2-hydroxymuconic semialdehyde hydrolase; No.9, KDK82_RS31095, 2-hydroxymuconic semialdehyde dehydrogenase; No.10, KDK82_RS31050, 4-oxalocrotonate tautomerase family protein; No.11, KDK82_RS31080, 2-oxo-3-hexenedioate decarboxylase; No.12, KDK82_RS31085, 2-oxopent-4-enoate hydratase; No.13, KDK82_RS31060, 4-hydroxy-2-oxovalerate aldolase; No.14, KDK82_RS31065, acetaldehyde dehydrogenase; No.1-1, KDK82_RS22950, catechol 1,2-dioxygenase; No.1-2, KDK82_RS22965, mandelate racemase; No.1-3,KDK82_RS22960, muconolactone delta-isomerase; No.1-4, KDK82_RS23310, 3-oxoadipate enol-lactonase; No.1-5, KDK82_RS01060, 3-oxoadipate CoA-transferase; No.1–6, KDK82_RS17030, acetyl-CoA C-acyltransferase