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Table 7 The penalty points for the determination of SF and VP for the reported methods and the proposed spectrophotometric method

From: Eco-friendly analytical methods for the determination of compounds with disparate spectral overlapping: application to antiviral formulation of sofosbuvir and velpatasvir

Reagents/instruments Penalty points
Proposed spectrophotometer method Reported HPLC method (Vanaja et al., 2018) Reported LC-MS/MS method (Elkady &Aboelwafa, 2018)
Methanol 12 18 18
Triethylamine - 6 -
Phosphate buffer - 0 -
Phosphoric acid - 2 -
Borate buffer 0 - -
MBTH - - -
NBD-Cl 0 - -
Formic acid - - 2
Acetonitrile - - 12
Acetone - - -
Ferric chloride 0 - -
UV-Vis spectrophotometer 0 - -
 HPLC - 0 -
 LC-MS - - 2
Occupational hazard 0 0 0
 Waste 6 6 8
Total penalty points Σ22 Σ32 Σ42
Analytical eco-scale
Total score
78 68 58
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