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Table 7 The penalty points for the determination of SF and VP for the reported methods and the proposed spectrophotometric method

From: Eco-friendly analytical methods for the determination of compounds with disparate spectral overlapping: application to antiviral formulation of sofosbuvir and velpatasvir

Reagents/instruments

Penalty points

Proposed spectrophotometer method

Reported HPLC method (Vanaja et al., 2018)

Reported LC-MS/MS method (Elkady &Aboelwafa, 2018)

Methanol

12

18

18

Triethylamine

-

6

-

Phosphate buffer

-

0

-

Phosphoric acid

-

2

-

Borate buffer

0

-

-

MBTH

-

-

-

NBD-Cl

0

-

-

Formic acid

-

-

2

Acetonitrile

-

-

12

Acetone

-

-

-

Ferric chloride

0

-

-

UV-Vis spectrophotometer

0

-

-

 HPLC

-

0

-

 LC-MS

-

-

2

Occupational hazard

0

0

0

 Waste

6

6

8

Total penalty points

Σ22

Σ32

Σ42

Analytical eco-scale

Total score

78

68

58