Reagents/instruments | Penalty points | ||
---|---|---|---|
Proposed spectrophotometer method | Reported HPLC method (Vanaja et al., 2018) | Reported LC-MS/MS method (Elkady &Aboelwafa, 2018) | |
Methanol | 12 | 18 | 18 |
Triethylamine | - | 6 | - |
Phosphate buffer | - | 0 | - |
Phosphoric acid | - | 2 | - |
Borate buffer | 0 | - | - |
MBTH | - | - | - |
NBD-Cl | 0 | - | - |
Formic acid | - | - | 2 |
Acetonitrile | - | - | 12 |
Acetone | - | - | - |
Ferric chloride | 0 | - | - |
UV-Vis spectrophotometer | 0 | - | - |
 HPLC | - | 0 | - |
 LC-MS | - | - | 2 |
Occupational hazard | 0 | 0 | 0 |
 Waste | 6 | 6 | 8 |
Total penalty points | Σ22 | Σ32 | Σ42 |
Analytical eco-scale Total score | 78 | 68 | 58 |