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Table 5 Comparison of the proposed method for SF and VP determination with other reported methods: Determination of SF in presence of VP

From: Eco-friendly analytical methods for the determination of compounds with disparate spectral overlapping: application to antiviral formulation of sofosbuvir and velpatasvir

Determination of SF in presence of VP
Point of comparison Proposed method Reference (Kamal et al., 2019) Reference (Rezk et al., 2019b) Reference (Attia et al., 2018)
Determination method Derivative ratio (2DD), delta absorbance (ΔA, Δ D1), and derivative compensation First derivative of ratio spectra (1 DD) and ratio difference spectrophotometry (RD) Dual wavelength (DW), ratio subtraction (RS), ratio difference (RD), and first derivative of ratio spectra method (1 DD) First derivative of SF spectra, calculated by Savitsky–Golay filters, at 251 nm
Linearity range (μg/mL) 5-50 5-50 5-90 4–40
LOD (μg/mL) (2DD) 0.471 (ΔA) 0.318 (Δ D1) 0.773 (1 DD) 0.150 (RD) 0.106 (DW) 1.21 (RS) 1.32 (RD) 1.19 (1 DD) 1.38 0.913
LOQ (μg/mL) (2DD) 1.568 (ΔA) 1.062 (Δ D1) 2.578 (1 DD) 0.454 (RD) 0.322 (DW) 3.67 (RS) 4.01 (RD) 3.61 (1 DD) 4.17 2.767
Applications Synthetic mixture, dosage form Determination of drugs in dosage form Laboratory prepared mixtures and dosage form Determination of drugs in pharmaceutical dosage form