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Table 2 Regression equation parameters of the spectrophotometric and spectrofluorimetric proposed methods for the determination of SF and VP

From: Eco-friendly analytical methods for the determination of compounds with disparate spectral overlapping: application to antiviral formulation of sofosbuvir and velpatasvir

Parameter

2DD

Δ A

Δ D1

NBD-Cl-UV

NBD-Cl-Flu

MBTH-UV

Wavelength (nm)

254

232

245

436

λem/ex 550/480

709

Linearity range (μg/mL)

5.00-50.00

5.00-50.00

5.00-50.00

5.00-500.00

0.50-20.00

5.00-150.00

Regression equation: Intercept (a)

0.009

-0.0105

-0.001

0.0083

416.26

0.0564

Slope (b)

0.0237

0.0265

0.0043

0.001

11.215

0.004

Correlation coefficient (r)

0.9996

0.9991

0.9992

0.9997

0.9997

0.9996

LOD (μg/mL)

0.471

0.318

0.773

0.87

0.08

1.03

LOQ (μg/mL)

1.568

1.062

2.578

2.87

0.27

3.44