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Table 2 Regression equation parameters of the spectrophotometric and spectrofluorimetric proposed methods for the determination of SF and VP

From: Eco-friendly analytical methods for the determination of compounds with disparate spectral overlapping: application to antiviral formulation of sofosbuvir and velpatasvir

Parameter 2DD Δ A Δ D1 NBD-Cl-UV NBD-Cl-Flu MBTH-UV
Wavelength (nm) 254 232 245 436 λem/ex 550/480 709
Linearity range (μg/mL) 5.00-50.00 5.00-50.00 5.00-50.00 5.00-500.00 0.50-20.00 5.00-150.00
Regression equation: Intercept (a) 0.009 -0.0105 -0.001 0.0083 416.26 0.0564
Slope (b) 0.0237 0.0265 0.0043 0.001 11.215 0.004
Correlation coefficient (r) 0.9996 0.9991 0.9992 0.9997 0.9997 0.9996
LOD (μg/mL) 0.471 0.318 0.773 0.87 0.08 1.03
LOQ (μg/mL) 1.568 1.062 2.578 2.87 0.27 3.44
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