A new high-performance liquid chromatographic method for the quality control of bioconverted Mori Folium extracts with appropriate marker compounds related to antidiabetes

Journal of Analytical Science and Technology

Table 1 Peak identification of five marker candidates in bioconverted MF extract by HPLC-DAD-ESI-MS/MS

Compound No.	Retention time (min)	UV λ (nm)	Molecular weight	Molecular formula	MS¹ (m/z)	MS² (Rel. Int. %)	Identification	CE (V)
1	17.67	217,323	180	C₉H₈O₄	[M-H]⁻ (178.95)	134.80 (100%), 133.90 (15%)	Trans-caffeic acid	17.0
2	30.51	217,309	182	C₇H₆O₄	[M + H]⁺ (183.05)	155.15 (38%), 123.10 (100%), 95.05 (71%), 77.00 (84%), 65.10 (23%)	Syringaldehyde	− 12.0
3	41.24	212,253,351	464	C₂₁H₂₀O₁₂	[M + H]⁺ (465.05)	303.00 (100%)	Morin-O-β-D-glucopyranoside	− 12.0
4	44.27	219,316	404	C₂₀H₂₀O₉	[M + HCOO]⁻ (449.15)	403.05 (50%), 240.90 (100%)	Moracin M-3′-O-β-glucopyranoside	20.0
5	53.57	214,265,339	448	C₂₁ H₂₀O₁₁	[M + H]⁺ (449.10)	284.90 (29%), 283.95 (100%), 254.90 (70%), 226.95 (69%)	Astragalin	− 13.0