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Table 2 Optimization by central composite design

From: Comprehensive stability-indicating method development of Avanafil Phosphodiesterase type 5 inhibitor using advanced Quality-by-Design approach

Run

A

B

C

D

Rs

(1–2)

Rs

(2–3)

Rs

(3–4)

Rs

(4–5)

Rs

(5–6)

Rs

(6–7)

1

60

4.5

1.25

20

3.571

4.200

16.690

1.200

3.770

0.000

2

60

4.5

0.75

20

3.780

4.950

8.160

1.830

4.323

0.110

3

70

3.5

0.5

30

4.890

1.420

8.800

0.000

0.000

0.200

4

50

5.5

1

30

1.810

3.650

14.630

2.770

2.200

1.860

5

50

5.5

1

10

1.620

3.050

8.220

5.710

3.090

2.680

6

60

2.5

0.75

20

0.000

2.110

0.600

11.100

0.200

4.100

7

50

3.5

0.5

10

1.460

3.790

1.430

1.550

3.320

2.260

8

60

4.5

0.75

20

4.770

5.110

17.530

3.250

1.630

4.450

9

70

3.5

1

10

5.120

1.720

27.140

0.800

8.380

0.000

10

70

5.5

1

10

1.320

1.660

10.710

5.160

1.180

25.00

11

70

5.5

1

30

43.160

1.660

2.041

0.560

0.000

0.100

12

60

4.5

0.75

20

4.740

5.100

17.210

3.140

1.420

4.420

13

60

4.5

0.75

0

2.100

4.590

11.600

2.780

1.290

2.600

14

40

4.5

0.75

20

4.180

11.00

4.015

1.900

6.590

1.820

15

60

4.5

0.25

20

5.150

6.240

23.300

1.800

4.600

0.000

16

70

3.5

1

30

14.750

6.640

1.520

7.920

1.011

4.790

17

60

4.5

0.75

20

5.350

6.440

23.500

1.840

4.610

0.500

18

80

4.5

0.75

20

6.140

1.630

2.510

0.000

0.000

0.950

19

50

5.5

0.5

30

1.510

2.320

15.490

3.007

3.389

0.300

20

50

3.5

1

10

1.920

1.220

4.090

1.110

5.950

1.880

21

70

5.5

0.5

10

1.520

11.990

5.380

1.380

16.00

4.310

22

50

3.5

1

30

1.410

3.320

4.160

6.570

2.370

2.001

23

60

4.5

0.75

20

4.670

5.190

17.330

3.140

1.480

4.310

24

50

3.5

0.5

30

1.220

4.420

1.260

8.710

2.920

0.100

25

70

5.5

0.5

30

0.000

5.070

5.410

32.720

2.650

4.000

26

60

4.5

0.75

40

4.760

5.400

20.400

2.650

1.710

0.800

27

50

5.5

0.5

10

1.990

4.000

10.060

7.280

3.740

2.800

28

60

6.5

0.75

20

0.000

22.160

2.600

3.041

2.000

0.100

29

60

4.5

0.75

20

4.760

5.098

17.410

3.150

1.420

4.410

30

70

3.5

0.5

10

0.000

0.000

3.130

2.330

0.000

0.000

  1. A = Mobile phase ratio, B = pH of buffer, C = Flow rate, and D = Temperature of column