Study of bioaccessibility of cobalt species in berries and seeds by mass spectrometry techniques

Journal of Analytical Science and Technology

Table 2 Cobalt species observed in enzymatic extracts analyzed by μ-HPLC-ESI-MS

Sample	Enzyme	Bioaccessibility (%)	t_r (min)	Molecular formula	(*m/z*)
açaí berries	pepsin	63.4	23.7	[Cbl + H]²⁺	665.2
			29.4	[CN-Cbl + 2H]²⁺	678.1
			27.3	[Me-Cbl + H]²⁺	673.2
			27.3	[Me-Cbl]⁺	1344.5
	pancreatin	94.1	28.9	[Cbl + H]²⁺	665.0
			25.7	[Ado-Cbl + H]²⁺	790.4
bilberries	pepsin	44.2	23.6	[Cbl + H]²⁺	665.1
			29.4	[CN-Cbl + 2H]²⁺	678.3
			29.4	[CN-Cbl + H]⁺	1355.5
			27.3	[Me-Cbl]⁺	1344.5
			25.7	[Ado-Cbl + H]²⁺	790.4
	pancreatin	32.1	28.7	[Cbl + H]²⁺	665.0
chia seeds	pepsin	56.1	29.3	[CN-Cbl + 2H]²⁺	678.7
			25.9	[Ado-Cbl + H]²⁺	790.3
	pancreatin	37.4	-	-	-
goji berries	pepsin	86.2	27.4	[Me-Cbl + H]²⁺	673.3
	pancreatin	91.4	28.8	[Cbl + H]²⁺	665.1
			27.3	[Me-Cbl + H]²⁺	673.0
standards
			23.6	[Cbl + H]²⁺	665.1
			27.2	[Me-Cbl]⁺/[Me-Cbl + H]⁺	637.2/1344.5
			25.7	[Ado-Cbl + H]²⁺	790.3
			29.4	[CN-Cbl +2H]⁺/[CN-Cbl + H]⁺	678.1/1355.5