Table 1 Operational parameters for HPLC, ICP-MS, and ESI-MS
From: Study of bioaccessibility of cobalt species in berries and seeds by mass spectrometry techniques
Settings | |
|---|---|
ICP-MS | Agilent 7500a |
RF Power | 1350 W |
Plasma, auxiliary, and nebulizer gas flow | 15.0, 1.0, and 1.05 L min−1 |
Cones | Sampler–Ni, Skimmer–Ni |
Monitored isotopes | 59Co |
Dwell time | 0.1 ms |
HPLC separation | |
Pump | Agilent 1100 |
Column | Superdex 200 (10 × 300 mm × 10 μm)—GE Healthcare Life Sciences |
Mobile phase | 10 mM ammonium acetate buffer (pH 7.4) |
Elution program | isocratic |
Flow | 0.7 mL min−1 |
Injection volume | 100 μL |
Column temperature | 24 °C |
Pump | Agilent 1200 Series |
Column | Zorbax SB C18 (5 μm, 4.6 × 150 mm) |
Mobile phase | A: mixture water-methanol (95:5 v/v) with 0.1 % formic acid B: mixture methanol-water (95:5 v/v) |
Injection volume [μL] | 0.2 |
Flow rate [μL min−1] | 5 |
Data acquisition mode | Time-resolved analysis (TRA) |
Integration time | 0.05–0.3 sec |
ESI-MS detection | |
Detector | Agilent 6460 Triple Quad LC/MS with JetStream technology |
Polarity | Positive, negative |
Mode | SCAN, PI |
Ionization voltage [V] | 1500–2500 (PI), 1500–2500 (NI) |
Nebulizer pressure [psi] | 45–55 |
Gas temperature [°C] | 300 |
Gas flow [L min−1] | 8 |
Sheath gas flow [L min−1] | 6–7 |
Sheath gas temperature [°C] | 300–350 |
Mass range, m/z | 100–1500 (with HPLC) |
Collision energy, V | 5–30 |