Skip to main content

Table 1 Operational parameters for HPLC, ICP-MS, and ESI-MS

From: Study of bioaccessibility of cobalt species in berries and seeds by mass spectrometry techniques

ICP-MS Agilent 7500a
 RF Power 1350 W
 Plasma, auxiliary, and nebulizer gas flow 15.0, 1.0, and 1.05 L min−1
 Cones Sampler–Ni, Skimmer–Ni
 Monitored isotopes 59Co
 Dwell time 0.1 ms
HPLC separation  
 Pump Agilent 1100
 Column Superdex 200 (10 × 300 mm × 10 μm)—GE Healthcare Life Sciences
 Mobile phase 10 mM ammonium acetate buffer (pH 7.4)
 Elution program isocratic
 Flow 0.7 mL min−1
 Injection volume 100 μL
 Column temperature 24 °C
 Pump Agilent 1200 Series
 Column Zorbax SB C18 (5 μm, 4.6 × 150 mm)
 Mobile phase A: mixture water-methanol (95:5 v/v) with 0.1 % formic acid
B: mixture methanol-water (95:5 v/v)
 Injection volume [μL] 0.2
 Flow rate [μL min−1] 5
 Data acquisition mode Time-resolved analysis (TRA)
 Integration time 0.05–0.3 sec
ESI-MS detection  
 Detector Agilent 6460 Triple Quad LC/MS with JetStream technology
 Polarity Positive, negative
 Mode SCAN, PI
 Ionization voltage [V] 1500–2500 (PI), 1500–2500 (NI)
 Nebulizer pressure [psi] 45–55
 Gas temperature [°C] 300
 Gas flow [L min−1] 8
 Sheath gas flow [L min−1] 6–7
 Sheath gas temperature [°C] 300–350
 Mass range, m/z 100–1500 (with HPLC)
 Collision energy, V 5–30