Table 3 Summary of stability results
Compound | Parameter | Benchtop stability (24 h) | Autosampler stability (192 h) | Freeze-thaw stability | Long-term stability | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
LQC (0.6 ng/mL) | HQC (160 ng/mL) | LQC (0.6 ng/mL) | HQC (160 ng/mL) | Cycles | LQC (0.6 ng/mL) | HQC (160 ng/mL) | Days | LQC (0.6 ng/mL) | HQC (160 ng/mL) | ||
EVT201 | Mean | 0.618 | 146 | 0.607 | 143 | 10 | 0.673 | 181 | 944 | 0.595 | 150 |
RE% | 3.0 | − 8.8 | 1.2 | − 10.6 | 12.2 | 13.1 | − 0.8 | − 6.3 | |||
SD | 0.005 | 3 | 0.011 | 4 | 0.068 | 8 | 0.057 | 2 | |||
CV% | 0.8 | 2.1 | 1.8 | 2.8 | 10.1 | 4.4 | 9.6 | 1.3 | |||
Ro46-1927 | Mean | 0.561 | 137 | 0.661 | 170 | 9 | 0.596 | 172 | 939 | 0.673 | 181 |
RE% | − 6.5 | − 14.4 | 10.2 | 6.3 | − 0.7 | 7.5 | 12.2 | 13.1 | |||
SD | 0.079 | 14 | 0.019 | 5 | 0.008 | 13 | 0.068 | 8 | |||
CV% | 14.1 | 10.2 | 2.9 | 2.9 | 1.3 | 7.6 | 10.1 | 4.4 | |||
Ro18-5528 | Mean | 0.557 | 139 | 0.622 | 144 | 10 | 0.529 | 153 | 694 | 0.593 | 172 |
RE% | − 7.2 | − 13.1 | 3.7 | − 10.0 | − 11.8 | − 4.4 | − 0.7 | 7.5 | |||
SD | 0.021 | 5 | 0.036 | 2 | 0.020 | 2 | 0.008 | 13 | |||
CV% | 3.8 | 3.6 | 5.8 | 1.4 | 3.8 | 1.3 | 1.3 | 7.6 | |||