Table 2 Comparison of matrix effect for EVT201 and its two metabolites in human urine with SPE and LLE
Extraction method | Compound | LQC (0.6 ng/mL) | HQC (160 ng/mL) | ||||
|---|---|---|---|---|---|---|---|
Mean of MF | Mean of ISMF | CV% of ISMF | Mean of MF | Mean of ISMF | CV% of ISMF | ||
SPE | EVT201 | 0.867 | 1.00 | 6.0 | 0.977 | 0.933 | 2.9 |
Ro46-1927 | 0.901 | 1.05 | 9.5 | 0.955 | 0.918 | 2.0 | |
Ro18-5528 | 0.860 | 0.995 | 7.9 | 0.977 | 0.939 | 3.0 | |
LLE | EVT201 | 0.981 | 0.962 | 5.3 | 0.949 | 0.981 | 1.9 |
Ro46-1927 | 1.02 | 1.00 | 4.4 | 0.974 | 1.01 | 3.0 | |
Ro18-5528 | 0.971 | 0.956 | 7.7 | 0.979 | 1.01 | 3.2 | |