Condition tested | LQC (15 ng/mL) | MQC (500 ng/mL) | HQC (8000 ng/mL) | ||||||
---|---|---|---|---|---|---|---|---|---|
Mean (ng/mL) | RSDa (%) | REb (%) | Mean (ng/mL) | RSDa (%) | REb (%) | Mean (ng/mL) | RSDa (%) | REb (%) | |
Mice | |||||||||
Control samples (freshly prepared) | – | 0.66 | – | – | 1.78 | – | – | 1,73 | – |
Freeze-thaw (− 80 °C, 3 cycles) | 15.45 | 2.68 | 3.01 | 466.17 | 2.82 | − 6.77 | 8205.57 | 2.08 | 2.57 |
Bench (RT, 24 h) | 14.76 | 3.70 | − 1.62 | 507.26 | 3.53 | 1.45 | 8456.82 | 0.58 | 5.71 |
Refrigerator (4 °C, 24 h) | 16.02 | 2.75 | 6.77 | 502.51 | 2.75 | 0.50 | 8368.14 | 0.43 | 4.60 |
Freezer (− 20 °C, 24 h) | 14.56 | 0.55 | − 2.94 | 486.76 | 1.29 | − 2.65 | 8195.62 | 2.09 | 2.45 |
Freezer (− 80 °C, 4 weeks) | 15.08 | 1.69 | 0.54 | 469.95 | 2.63 | − 6.01 | 7988.80 | 0.32 | − 0.14 |
Post-preparative (RT, 24 h) | 14.74 | 0.86 | − 1.76 | 504.12 | 4.51 | 0.82 | 8319.10 | 1.62 | 3.99 |
Post-preparative (4 °C, 24 h) | 15.35 | 0.32 | 2.36 | 466.07 | 2.43 | − 6.79 | 7845.21 | 1.35 | − 1.93 |
Post-preparative (4 °C, 1 week) | 14.54 | 0.76 | − 3.04 | 484.56 | 1.11 | − 3.09 | 7868.35 | 0.58 | − 1.65 |
Human | |||||||||
Control samples (freshly prepared) | – | 9.51 | – | – | 4.15 | – | – | 0.22 | – |
Freeze-thaw (− 80 °C, 3 cycles) | 15.30 | 1.53 | 1.98 | 528.25 | 0.40 | 5.65 | 8264.74 | 0.65 | 3.31 |
Bench (RT, 24 h) | 14.24 | 2.39 | − 5.08 | 481.51 | 0.95 | − 3.70 | 8377.79 | 0.82 | 4.72 |
Refrigerator (4 °C, 24 h) | 15.20 | 0.14 | 1.37 | 483.95 | 3.23 | − 3.21 | 8368.67 | 1.98 | 4.61 |
Freezer (− 20 °C, 24 h) | 15.23 | 1.54 | 1.55 | 531.76 | 1.03 | 6.35 | 7999.97 | 0.87 | 0.00 |
Freezer (− 80 °C, 4 weeks) | 14.82 | 2.07 | − 1.17 | 540.29 | 1.39 | 8.06 | 8002.13 | 5.89 | 0.03 |
Post-preparative (RT, 24 h) | 14.85 | 1.49 | − 1.02 | 550.94 | 3.75 | 10.19 | 8335.96 | 1.60 | 4.20 |
Post-preparative (4 °C, 24 h) | 14.85 | 3.49 | − 1.01 | 488.57 | 1.68 | − 2.29 | 8307.94 | 1.05 | 3.85 |
Post-preparative (4 °C, 1 week) | 15.37 | 1.68 | 2.50 | 470.24 | 2.17 | − 5.95 | 8167.04 | 0.99 | 2.09 |