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Table 1 Analytical characteristics of the established method for gastric fluid samples

From: UHPLC-MS/MS profiling of histidine and bile acid metabolism in human gastric fluid for diagnosis of gastric diseases

Analytes Calibration range (ng/mL) Linear regression equation r2 LODs (ng/mL) LOQs (ng/mL) Spiked concentration (ng/mL) Intra-day assay* (%) Inter-day assay* (%) Recovery* (%)
His 250–10000 y = 0.0008x−0.2514 0.9955 182.97 250 500 109.45 ± 10.70 104.31 ± 2.20 90.51 ± 11.03
2500 98.05 ± 4.88 98.65 ± 2.32 91.47 ± 10.22
8000 100.41 ± 7.32 99.80 ± 2.85 93.32 ± 8.78
HIST 25–1000 y = 0.0006x−0.0348 0.9938 8.03 25 50 109.56 ± 10.08 92.66 ± 1.89 91.36 ± 7.56
250 98.69 ± 3.01 99.77 ± 2.18 102.04 ± 6.04
800 101.09 ± 8.26 98.00 ± 2.11 104.13 ± 5.32
CA 25–800 y = 0.0027x−0.0591 0.9986 17.79 25 50 87.34 ± 13.04 113.31 ± 11.67 87.86 ± 13.18
250 104.19 ± 5.78 93.61 ± 2.40 106.96 ± 6.42
800 99.60 ± 12.09 100.59 ± 2.78 99.25 ± 4.44
UDCA 25–1000 y = 0.0255x−0.4858 0.9981 3.84 25 50 106.04 ± 5.16 96.03 ± 11.41 85.75 ± 12.97
250 97.57 ± 6.19 98.58 ± 5.45 97.28 ± 14.61
800 99.43 ± 5.86 97.73 ± 2.30 107.07 ± 12.47
CDCA 25–1000 y = 0.0021x−0.0378 0.9980 2.32 25 50 99.79 ± 8.07 103.04 ± 8.87 92.35 ± 14.08
250 98.91 ± 3.39 102.43 ± 10.56 87.17 ± 9.94
800 99.13 ± 9.54 102.68 ± 7.79 99.63 ± 7.92
DCA 25–1000 y = 0.0258x−0.5951 0.9981 5.97 25 50 94.92 ± 10.90 88.59 ± 7.73 91.26 ± 14.49
250 101.91 ± 10.72 106.79 ± 2.83 85.85 ± 12.10
800 100.09 ± 7.09 99.66 ± 7.14 101.23 ± 12.37
LCA 25–1000 y = 0.0045x−0.1169 0.9984 6.82 25 50 98.45 ± 8.01 90.93 ± 9.74 88.63 ± 7.88
250 99.50 ± 5.73 102.64 ± 6.58 95.94 ± 9.46
800 99.17 ± 4.08 99.46 ± 12.10 101.12 ± 7.21
  1. *Recovery, precision, and accuracy were determined in 6 replicates