Table 1 Analytical characteristics of the established method for gastric fluid samples
Analytes | Calibration range (ng/mL) | Linear regression equation | r2 | LODs (ng/mL) | LOQs (ng/mL) | Spiked concentration (ng/mL) | Intra-day assay* (%) | Inter-day assay* (%) | Recovery* (%) |
|---|---|---|---|---|---|---|---|---|---|
His | 250–10000 | y = 0.0008x−0.2514 | 0.9955 | 182.97 | 250 | 500 | 109.45 ± 10.70 | 104.31 ± 2.20 | 90.51 ± 11.03 |
2500 | 98.05 ± 4.88 | 98.65 ± 2.32 | 91.47 ± 10.22 | ||||||
8000 | 100.41 ± 7.32 | 99.80 ± 2.85 | 93.32 ± 8.78 | ||||||
HIST | 25–1000 | y = 0.0006x−0.0348 | 0.9938 | 8.03 | 25 | 50 | 109.56 ± 10.08 | 92.66 ± 1.89 | 91.36 ± 7.56 |
250 | 98.69 ± 3.01 | 99.77 ± 2.18 | 102.04 ± 6.04 | ||||||
800 | 101.09 ± 8.26 | 98.00 ± 2.11 | 104.13 ± 5.32 | ||||||
CA | 25–800 | y = 0.0027x−0.0591 | 0.9986 | 17.79 | 25 | 50 | 87.34 ± 13.04 | 113.31 ± 11.67 | 87.86 ± 13.18 |
250 | 104.19 ± 5.78 | 93.61 ± 2.40 | 106.96 ± 6.42 | ||||||
800 | 99.60 ± 12.09 | 100.59 ± 2.78 | 99.25 ± 4.44 | ||||||
UDCA | 25–1000 | y = 0.0255x−0.4858 | 0.9981 | 3.84 | 25 | 50 | 106.04 ± 5.16 | 96.03 ± 11.41 | 85.75 ± 12.97 |
250 | 97.57 ± 6.19 | 98.58 ± 5.45 | 97.28 ± 14.61 | ||||||
800 | 99.43 ± 5.86 | 97.73 ± 2.30 | 107.07 ± 12.47 | ||||||
CDCA | 25–1000 | y = 0.0021x−0.0378 | 0.9980 | 2.32 | 25 | 50 | 99.79 ± 8.07 | 103.04 ± 8.87 | 92.35 ± 14.08 |
250 | 98.91 ± 3.39 | 102.43 ± 10.56 | 87.17 ± 9.94 | ||||||
800 | 99.13 ± 9.54 | 102.68 ± 7.79 | 99.63 ± 7.92 | ||||||
DCA | 25–1000 | y = 0.0258x−0.5951 | 0.9981 | 5.97 | 25 | 50 | 94.92 ± 10.90 | 88.59 ± 7.73 | 91.26 ± 14.49 |
250 | 101.91 ± 10.72 | 106.79 ± 2.83 | 85.85 ± 12.10 | ||||||
800 | 100.09 ± 7.09 | 99.66 ± 7.14 | 101.23 ± 12.37 | ||||||
LCA | 25–1000 | y = 0.0045x−0.1169 | 0.9984 | 6.82 | 25 | 50 | 98.45 ± 8.01 | 90.93 ± 9.74 | 88.63 ± 7.88 |
250 | 99.50 ± 5.73 | 102.64 ± 6.58 | 95.94 ± 9.46 | ||||||
800 | 99.17 ± 4.08 | 99.46 ± 12.10 | 101.12 ± 7.21 |