Table 1 Ion pair and MS parameter for the analysis of the five analytes and the internal standard in negative ion ESI mode
Ion pair(m/z) | Declustering potential (V) | Collision energy (V) | Retention time (min) | |
|---|---|---|---|---|
Gastrodin | 331.2→123.0 | − 23.0 | − 19.0 | 1.96 |
Parishin A | 995.5→727.0 | − 26.9 | − 25.0 | 7.10 |
Parishin B | 727.4→160.9 | − 5.07 | − 41.7 | 6.61 |
Parishin C | 727.0→161.1 | − 20.0 | − 42.9 | 6.81 |
Parishin E | 459.2→110.8 | − 48.8 | − 29.3 | 5.43 |
Bergenin (IS) | 327.0→192.0 | − 115.0 | − 27.9 | 5.31 |