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Table 4 Stability data for bromophenol-thiosemicarbazone hybrid in rat plasma (n = 3).

From: An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study

Nominal conc. (nmol/L)

100

1000

10000

On bench top at room temperature for 3 h

Conc. (nmol/L)

93.6 ± 14.2

1139 ± 36

11196 ± 622

Stability (%)

93.6

114

112

In autosampler maintained at 6 °C for 24 h

Conc. (nmol/L)

97.8 ± 15.6

1128 ± 63.6

12023 ± 1112

Stability (%)

97.8

113

120

After 3 freeze/thaw cycles of − 70 ↔ 23 °C

Conc. (nmol/L)

98.8 ± 3.1

1095 ± 32

10965 ± 683

Stability (%)

98.8

110

110

Short term stability ( − 70 °C for 3 days)

Conc.(nmol/L)

84.1 ± 7.4

1122 ± 7

11230 ± 563

Stability (%)

84.1

112

112

Long term stability ( − 70 °C for 30 days)

Conc.(nmol/L)

92.0 ± 4.6

1095 ± 78

10626 ± 768

Stability (%)

90.3

113

112