FIA-SIM method | Internal standard method | ||||
---|---|---|---|---|---|
Gentamicin | Tobramycin | Amikacin | Gentamicin | Tobramycin | |
Selected m/z | 322 | 324 | 425 | 322 | 324 |
Internal standard (m/z) | – | – | – | Tobramycin (324) | Gentamicin (322) |
Limit of detection (ng/mL) | 1 | 5 | 1 | 1 | 5 |
Linear dynamic range (ng/mL) | 10–1000 | 25–2500 | 10–1000 | 10–1000 | 25–2500 |
RSD intra-day, n = 5 (%) | 6.1 | 5.2 | 11.7 | 2.2 | 2.9 |
RSD Inter-day, n = 15 (%) | 8.0 | 7.8 | – | 3.5 | 3.8 |
Concentration in commercial sample (mg/mL) | 41.3 ± 2.2 | 42.2 ± 1.9 | – | 40.5 ± 1.2 | 41.0 ± 0.9 |