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Table 1 Summary of flow-injection analysis single-ion monitoring (FIA-SIM) method versus the internal standard method

From: Rapid determination of aminoglycosides in pharmaceutical preparations by electrospray ionization mass spectrometry

 

FIA-SIM method

Internal standard method

 

Gentamicin

Tobramycin

Amikacin

Gentamicin

Tobramycin

Selected m/z

322

324

425

322

324

Internal standard (m/z)

Tobramycin (324)

Gentamicin (322)

Limit of detection (ng/mL)

1

5

1

1

5

Linear dynamic range (ng/mL)

10–1000

25–2500

10–1000

10–1000

25–2500

RSD intra-day, n = 5 (%)

6.1

5.2

11.7

2.2

2.9

RSD Inter-day, n = 15 (%)

8.0

7.8

3.5

3.8

Concentration in commercial sample (mg/mL)

41.3 ± 2.2

42.2 ± 1.9

40.5 ± 1.2

41.0 ± 0.9