Simultaneous chromatographic analysis of Sofosbuvir/Ledipasvir in their combined dosage form: an application to green analytical chemistry

Journal of Analytical Science and Technology

Table 7 Results of statistical comparison^a between the developed chromatographic methods and reported HPLC method (Rote et al. 2017) used for the simultaneous determination of SBR and LPV

Parameters	Proposed HPTLC-densitometric method		Proposed RP HPLC-UV method		In-house HPLC method^b
Parameters	SBR	LPV	SBR	LPV	SBR	LPV
Mean	99.78	100.04	99.87	99.64	100.90	100.08
SD	0.970	1.090	1.270	1.190	0.835	0.553
Variance	0.941	1.188	1.613	1.416	0.697	0.306
t test^a	1.957	0.073	1.515	0.750	–
F-test^a	1.349	3.885	2.313	4.631	–

^aThe theoretical values of t and F at P = 0.05 are (2.306) and (6.388), respectively where n = 5
^bRP-HPLC method was performed on the Systronics isocratic HPLC system equipped with SP930 D HPLC pump and dual wavelength UV-VIS detector and C18 column (250 mm × 4.6 mm, 5 μm), using the mobile phase (Methanol: Water 83:17 v/v) pH 3.0 with 0.05% acidic acid at a flow rate of 1.0 ml/min, injection volume 20 μl and UV detection at 245 nm