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Table 7 Results of statistical comparisona between the developed chromatographic methods and reported HPLC method (Rote et al. 2017) used for the simultaneous determination of SBR and LPV

From: Simultaneous chromatographic analysis of Sofosbuvir/Ledipasvir in their combined dosage form: an application to green analytical chemistry

ParametersProposed HPTLC-densitometric methodProposed RP HPLC-UV methodIn-house HPLC methodb
SBRLPVSBRLPVSBRLPV
Mean99.78100.0499.8799.64100.90100.08
SD0.9701.0901.2701.1900.8350.553
Variance0.9411.1881.6131.4160.6970.306
t testa1.9570.0731.5150.750
F-testa1.3493.8852.3134.631
  1. aThe theoretical values of t and F at P = 0.05 are (2.306) and (6.388), respectively where n = 5
  2. bRP-HPLC method was performed on the Systronics isocratic HPLC system equipped with SP930 D HPLC pump and dual wavelength UV-VIS detector and C18 column (250 mm × 4.6 mm, 5 μm), using the mobile phase (Methanol: Water 83:17 v/v) pH 3.0 with 0.05% acidic acid at a flow rate of 1.0 ml/min, injection volume 20 μl and UV detection at 245 nm