Simultaneous chromatographic analysis of Sofosbuvir/Ledipasvir in their combined dosage form: an application to green analytical chemistry

Journal of Analytical Science and Technology

Table 5 Simultaneous estimation of SBR and LPV in Heterosofir Plus® tablets using the adopted chromatographic methods

HPTLC-densitometric method
Pharmaceutical preparation	Sample no.	Taken amount (μg band^− 1)		Found^a amount (μg band^− 1)		% Recovery
Pharmaceutical preparation	Sample no.	SBR	LPV	SBR	LPV	SBR	LPV
Heterosofir Plus® tablets labeled to contain 400 mg SRB + 90 mg LPV per tablet	1	4.00	0.90	4.02	0.90	100.50	100.00
	2	8.00	1.80	8.10	1.79	101.25	99.44
	3	16.00	3.60	15.92	3.62	99.50	100.56
	Mean ± % RSD					100.42 ± 0.878	100.00 ± 0.556
RP HPLC-UV method
Pharmaceutical preparation	Sample no.	Taken amount (μg.ml^− 1)		Found^a amount (μg.ml^− 1)		% Recovery
Pharmaceutical preparation	Sample no.	SBR	LPV	SBR	LPV	SBR	LPV
Heterosofir Plus® tablets labeled to contain 400 mg SRB + 90 mg LPV per tablet	1	20.00	4.50	20.14	4.51	100.70	100.22
	2	40.00	9.00	40.03	8.83	100.08	98.11
	3	80.00	18.00	79.52	17.92	99.40	99.56
	Mean ± % RSD					100.06 ± 0.650	99.30 ± 1.079