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Table 1 Parameters of system suitability testing of the adopted chromatographic methods for the simultaneous determination of SBR and LPV

From: Simultaneous chromatographic analysis of Sofosbuvir/Ledipasvir in their combined dosage form: an application to green analytical chemistry

Parameters

SBR

LPV

Reference value

HPTLC-densitometric method

 Retardation factor (Rf)

0.38

0.61

 

 Resolutiona (Rs)

4.09 a

Rs ≥ 2

 Tailing factor (T)

0.79

0.56

T ≤ 2

 Capacity Factor (K′)

6.25

7.62

1 < K′ < 10

 Selectivity (α)

3.61a

α > 1

RP HPLC-UV method

 Retention time (Rt; min)

3.03

4.07

 

 Resolutiona (Rs)

3.58

ـ

Rs ≥ 2

 Tailing factor (T)

1.49

1.26

T ≤ 2

 Asymmetry factor (Af)

1.04

1.00

0.9 < Af < 1.1

 Capacity Factor (K′)

1.32

2.12

1 < K′ < 10

 Selectivity (α)

1.60a

α > 1

 Injection repeatabilityb

0.83

0.75

RSD ≤ 1% for n ≥ 6

 Theoretical plates (N)

3936

2108

N > 2000

 Height equivalent to theoretical plate (HETP; cm plate− 1)

0.0038

0.0071

The smaller the value, the higher the column efficiency

  1. aTo LPV
  2. bRSD% for six injections