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Table 1 Parameters of system suitability testing of the adopted chromatographic methods for the simultaneous determination of SBR and LPV

From: Simultaneous chromatographic analysis of Sofosbuvir/Ledipasvir in their combined dosage form: an application to green analytical chemistry

ParametersSBRLPVReference value
HPTLC-densitometric method
 Retardation factor (Rf)0.380.61 
 Resolutiona (Rs)4.09 aRs ≥ 2
 Tailing factor (T)0.790.56T ≤ 2
 Capacity Factor (K′)6.257.621 < K′ < 10
 Selectivity (α)3.61aα > 1
RP HPLC-UV method
 Retention time (Rt; min)3.034.07 
 Resolutiona (Rs)3.58ـRs ≥ 2
 Tailing factor (T)1.491.26T ≤ 2
 Asymmetry factor (Af)1.041.000.9 < Af < 1.1
 Capacity Factor (K′)1.322.121 < K′ < 10
 Selectivity (α)1.60aα > 1
 Injection repeatabilityb0.830.75RSD ≤ 1% for n ≥ 6
 Theoretical plates (N)39362108N > 2000
 Height equivalent to theoretical plate (HETP; cm plate− 1)0.00380.0071The smaller the value, the higher the column efficiency
  1. aTo LPV
  2. bRSD% for six injections