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Table 4 HPLC analysis conditions of AI 1~3

From: In vitro and in vivo anti-aging effects of compounds isolated from Artemisia iwayomogi

Detector

UV (280 nm)

Column

AKZO NOBEL. KR100-5C18 (4.6 × 250 mm)

Mobile phase

A—Water; B—CH3CN

Time

A (%)

B (%)

0

70

30

30

70

30

40

30

70

45

70

30

60

70

30

Flow rate

1.0 mL/min

Injection volume

20 μL