Method development for the quantitative determination of short chain fatty acids in microbial samples by solid phase extraction and gas chromatography with flame ionization detection

Journal of Analytical Science and Technology

Table 1 Calibration, LOD, and LOQ of SCFAs obtained by GC-FID

Compound	Calibration curve			LOD (μg/mL)	LOQ (μg/mL)
Compound	Slope	Intercept	Correlation coefficient	LOD (μg/mL)	LOQ (μg/mL)
Acetic acid	0.0088	0.3388	0.9994	3.00	9.09
Propionic acid	0.0241	0.1869	0.9992	3.70	11.20
Butyric acid	0.0333	0.1061	0.9998	1.88	5.71
Valeric acid	0.0331	0.1415	0.9995	2.79	8.46