Storage conditions | Added conc. (μg/mL) | Calculated conc. (μg/mL) (mean ± S.D) | RE (%) |
---|---|---|---|
Ambient (6 h) | 0.05 | 0.07 ± 1.92 | 2.4 |
0.1 | 0.12 ± 2.34 | 2.9 | |
2.0 | 1.95 ± 3.02 | 2.5 | |
5.0 | 2.95 ± 3.01 | 2.3 | |
7.5 | 7.52 ± 3.83 | 3.5 | |
3 freeze (− 20 °C) thaw cycles | 0.05 | 0.04 ± 3.07 | 2.1 |
0.1 | 0.09 ± 4.41 | 2.6 | |
2.0 | 2.05 ± 3.80 | 3.1 | |
5.0 | 2.90 ± 3.02 | 2.4 | |
7.5 | 7.48 ± 4.29 | 4.2 | |
2–10 °C, 3 days | 0.05 | 0.06 ± 3.54 | 2.9 |
0.1 | 0.13 ± 4.07 | 3.8 | |
2.0 | 1.96 ± 3.45 | 2.4 | |
5.0 | 2.90 ± 3.07 | 2.4 | |
7.5 | 7.42 ± 4.21 | 3.7 | |
Autosampler stability (24 h) | 0.05 | 0.08 ± 2.90 | 3.2 |
0.1 | 0.15 ± 3.42 | 2.9 | |
2.0 | 2.10 ± 2.61 | 2.2 | |
5.0 | 2.91 ± 3.03 | 2.4 | |
7.5 | 7.44 ± 4.22 | 3.8 |