Table 2 Partition ratio and recovery (%) of daclatasvir a at different IL:ACN ratios and NaCl concentrations
Conditions | Concentration of daclatasvir (mM) | Peak area | Partition ratio (p) | Recovery (%) | ||
|---|---|---|---|---|---|---|
IL | Serum | IL | Serum | |||
IL:ACN (v/v) | ||||||
0.11 | 0.0435 | 0.0301 | 1151275 | 1005199 | 1.447 | 52.7 |
0.25 | 0.0524 | 0.0245 | 1426919 | 724542 | 2.138 | 65.9 |
0.43 | 0.0720 | 0.0022 | 2118122 | 30648 | 32.72 | 98.8 |
0.67 | 0.0627 | 0.0175 | 1703875 | 447811 | 3.582 | 80.6 |
1.00 | 0.0560 | 0.0195 | 1593382 | 559283 | 2.971 | 75.2 |
NaCl (%) | ||||||
1.0 | 0.0817 | 0.0010 | 2129955 | 20739 | 81.7 | 99.1 |
2.0 | 0.0745 | 0.0008 | 2127401 | 15840 | 93.1 | 99.0 |
5.0 | 0.0712 | 0.0006 | 2130342 | 10811 | 118.6 | 99.5 |
10.0 | 0.0704 | 0.0006 | 2130342 | 10811 | 118.6 | 99.5 |