S. No | Parameters varied | Condition | Retention time (min) |
---|---|---|---|
1 | Injection volume (μl) | 20 | 7.097 |
10 | 7.22 | ||
Mean | 7.1585 | ||
SD | 0.086974134 | ||
% RSD | 1.214977077 | ||
2 | Flow rate (ml/min) | 0.6 | 7 |
0.8 | 6.8 | ||
1 | 7 | ||
Mean | 6.9 | ||
SD | 0.141421356 | ||
% RSD | 2.049584873 | ||
3 | Mobile phase ratio | Ratio (60:40) | 6.8 |
Ratio (70:30) | 7 | ||
Mean | 6.9 | ||
SD | 0.141421356 | ||
% RSD | 2.049584873 | ||
4 | Column temp (°C) | 35 °C | 7 |
30 ° C | 7.2 | ||
Mean | 7.1 | ||
SD | 0.141421356 | ||
% RSD | 1.991850088 | ||
5 | pH of mobile phase | 1% OPA (pH 2.27) | 7.05 |
0.1% OPA (pH 2.97) | 7.084 | ||
0.5% OPA (pH 2.47) | 7.22 | ||
Mean | 7.152 | ||
SD | 0.096166522 | ||
% RSD | 1.34461021 |