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Table 7 Effect of variation in the parameters on the retention time

From: Development of a new, sensitive, and robust analytical and bio-analytical RP-HPLC method for in-vitro and in-vivo quantification of naringenin in polymeric nanocarriers

S. No Parameters varied Condition Retention time (min)
1 Injection volume (μl) 20 7.097
  10 7.22
  Mean 7.1585
  SD 0.086974134
  % RSD 1.214977077
2 Flow rate (ml/min) 0.6 7
  0.8 6.8
  1 7
  Mean 6.9
  SD 0.141421356
  % RSD 2.049584873
3 Mobile phase ratio Ratio (60:40) 6.8
  Ratio (70:30) 7
  Mean 6.9
  SD 0.141421356
  % RSD 2.049584873
4 Column temp (°C) 35 °C 7
  30 ° C 7.2
  Mean 7.1
  SD 0.141421356
  % RSD 1.991850088
5 pH of mobile phase 1% OPA (pH 2.27) 7.05
  0.1% OPA (pH 2.97) 7.084
  0.5% OPA (pH 2.47) 7.22
  Mean 7.152
  SD 0.096166522
  % RSD 1.34461021