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Table 7 Effect of variation in the parameters on the retention time

From: Development of a new, sensitive, and robust analytical and bio-analytical RP-HPLC method for in-vitro and in-vivo quantification of naringenin in polymeric nanocarriers

S. No

Parameters varied

Condition

Retention time (min)

1

Injection volume (μl)

20

7.097

 

10

7.22

 

Mean

7.1585

 

SD

0.086974134

 

% RSD

1.214977077

2

Flow rate (ml/min)

0.6

7

 

0.8

6.8

 

1

7

 

Mean

6.9

 

SD

0.141421356

 

% RSD

2.049584873

3

Mobile phase ratio

Ratio (60:40)

6.8

 

Ratio (70:30)

7

 

Mean

6.9

 

SD

0.141421356

 

% RSD

2.049584873

4

Column temp (°C)

35 °C

7

 

30 ° C

7.2

 

Mean

7.1

 

SD

0.141421356

 

% RSD

1.991850088

5

pH of mobile phase

1% OPA (pH 2.27)

7.05

 

0.1% OPA (pH 2.97)

7.084

 

0.5% OPA (pH 2.47)

7.22

 

Mean

7.152

 

SD

0.096166522

 

% RSD

1.34461021