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Journal of Analytical Science and Technology

Table 1 Organic acid analysis of edible food plants and milk under E. coli treatments (0 h, 24 h, and 72 h) using GC-MS

From: Microbial production of dicarboxylic acids from edible plants and milk using GC-MS

Species and family

Common name and edible part

E. coli treatment (h)

Citric acid (mg/g DW)

Succinic acid (mg/g DW)

Fumaric acid (mg/g DW)

Malic acid (mg/g DW)

Source of variation

F-ratios

HSD

Triticum aestivum L.

Wheat grain

0

1.97 ± 0.24

0.46 ± 0.08

0.29 ± 0.03

0.93 ± 0.27

Acids

183

7.09

Fam. Poaceae

24

1.39 ± 0.10

32.48 ± 5.35

0.27 ± 0.05

0.81 ± 0.20

Time

49

 

72

1.28 ± 0.09

45.12 ± 8.05

0.27 ± 0.005

0.58 ± 0.10

Interaction

52

 

Zea mays L.

Maize/corn grain

0

4.56 ± 0.36

0.47 ± 0.10

0.22 ± 0.07

0.59 ± 0.10

Acids

157

3.28

Fam. Poaceae

24

1.29 ± 0.02

4.21 ± 0.79

0.23 ± 0.05

0.59 ± 0.08

Time

107

 

72

1.40 ± 0.29

30.77 ± 4.50

0.26 ± 0.04

0.61 ± 0.07

Interaction

126

 

Vigna mungo (L.)

Urad bean/Maah seeds

0

19.71 ± 3.68

0.72 ± 0.03

0.24 ± 0.05

0.86 ± 0.14

Acids

237

5.64

Hepper

24

ND

30.21 ± 5.82

0.35 ± 0.05

0.87 ± 0.14

Time

68

 

Fam. Fabaceae

72

1.42 ± 0.23

34.10 ± 5.21

2.88 ± 0.96

0.83 ± 0.23

Interaction

59

 

Lens culinaris Medicus

Lentil/Masoor seeds

0

10.44 ± 2.59

5.40 ± 0.88

0.24 ± 0.08

0.84 ± 0.19

Acids

253

7.08

Fam. Fabaceae

24

1.80 ± 0.30

48.24 ± 8.24

1.56 ± 0.24

0.72 ± 0.08

Time

33

 

72

1.36 ± 0.31

43.93 ± 5.48

0.24 ± 0.07

1.30 ± 0.19

Interaction

59

 

Pisum sativum L.

Peas seeds

0

82.43 ± 17.34

1.81 ± 0.04

0.76 ± 0.004

5.39 ± 0.63

Acids

59

3.69

Fam. Fabaceae

24

5.14 ± 1.05

3.87 ± 0.04

1.35 ± 0.042

2.32 ± 0.50

Time

41

 

72

4.67 ± 1.13

35.28 ± 5.93

0.80 ± 0.004

3.07 ± 0.67

Interaction

70

 

Phaseolus vulgaris L.

Kidney beans/Rajmaah seeds

0

6.82 ± 1.80

1.75 ± 0.28

0.22 ± 0.069

1.45 ± 0.10

Acids

121

5.23

Fam. Fabaceae

24

11.41 ± 1.94

2.02 ± 0.16

0.37 ± 0.092

0.60 ± 0.11

Time

78

 

72

1.50 ± 0.26

45.74 ± 6.72

0.29 ± 0.058

0.72 ± 0.08

Interaction

133

 

Cicer arietinum L.

Chickpea/black gram seeds

0

4.90 ± 0.65

23.22 ± 3.71

0.22 ± 0.02

Nd

Acids

291

3.71

var. black gram

24

1.31 ± 0.19

25.67 ± 5.48

0.23 ± 0.05

Nd

Time

255

 

Fam. Fabaceae var.

72

1.32 ± 0.25

81.81 ± 7.93

0.22 ± 0.03

Nd

Interaction

274

 

Cicer arietinum L.

Chickpea/white gram/Kabuli gram seeds

0

21.72 ± 4.47

42.31 ± 5.21

0.22 ± 0.03

0.60 ± 0.09

Acids

362

5.37

var. White gram

24

1.50 ± 0.35

24.24 ± 2.64

0.54 ± 0.05

0.59 ± 0.04

Time

93

 

Fam. Fabaceae

72

1.47 ± 0.24

19.44 ± 3.09

0.44 ± 0.06

0.60 ± 0.10

Interaction

33

 

Solanum tuberosum L.

Potato tubers

0

6.06 ± 1.16

3.01 ± 0.19

0.87 ± 0.19

2.43 ± 0.53

Acids

2844

0.110

Fam. Solanaceae

24

5.67 ± 0.77

18.82 ± 2.91

0.97 ± 0.24

2.47 ± 0.005

Time

1985

 

72

5.38 ± 0.97

2.43 ± 0.48

0.92 ± 0.29

2.43 ± 0.44

Interaction

1969

 

Solanum lycopersicum L.

Tomato fruits

0

77.72 ± 11.7

52.73 ± 11.04

2.50 ± 0.92

6.71 ± 0.92

Acids

144

3.50

Fam. Solanaceae

24

204.09 ± 29.1

275.36 ± 44.7

2.50 ± 0.65

6.58 ± 1.18

Time

153

 

72

27.88 ± 4.20

35.37 ± 6.57

2.50 ± 0.65

6.58 ± 1.05

Interaction

56

 

Brassica oleracea.

White cauliflower inflorescence

0

16.22 ± 1.31

2.17 ± 0.05

2.11 ± 0.006

9.99 ± 1.78

Acids

920

0.358

var. botrytis L.

24

6.79 ± 1.08

35.80 ± 0.45

1.08 ± 0.011

2.97 ± 0.05

Time

199

 

Fam. Brassicaceae

72

6.74 ± 1.14

14.62 ± 0.17

1.08 ± 0.40

2.91 ± 0.22

Interaction

626

 

Malus pumila Miller

Apple mesocarp of the fruit

0

5.81 ± 0.45

1.89 ± 0.05

0.97 ± 0.25

2.70 ± 1.73

Acids

121

2.34

Fam. Rosaceae

24

Nd

44.83 ± 7.85

0.97 ± 0.35

2.96 ± 0.51

Time

32

 

72

Nd

36.11 ± 9.28

0.97 ± 0.005

3.01 ± 0.51

Interaction

32

 

Musa paradisiaca

Banana mesocarp of the fruit

0

15.16 ± 2.64

8.55 ± 1.17

0.70 ± 0.18

5.87 ± 0.44

Acids

451

7.93

Colla

24

87.71 ± 18.05

207.58 ± 17.5

0.66 ± 0.11

2.06 ± 0.36

Time

152

 

Fam. Musaceae

72

4.07 ± 0.40

233.01 ± 25.6

0.70 ± 0.22

2.75 ± 0.22

Interaction

125

 

Vitis vinifera L.

Grapes Fruits

0

24.31 ± 5.19

5.66 ± 0.94

2.60 ± 0.82

34.46 ± 6.01

Acids

345

5.88

Fam. Vitaceae

24

12.86 ± 2.12

504.57 ± 57.46

2.24 ± 0.59

7.20 ± 1.41

Time

83

 

72

21.71 ± 2.24

409.58 ± 58.17

2.24 ± 0.11

26.08 ± 1.18

Interaction

99

 

Carica papaya L.

Papaya fruit

0

175.68 ± 48.02

3.70 ± 0.81

1.33 ± 0.37

8.58 ± 1.48

Acids

259

11.31

Fam. Caricaceae

24

54.32 ± 12.21

715.8 ± 78.21

1.41 ± 0.44

5.62 ± 0.07

Time

86

 

72

73.70 ± 17.24

262.5 ± 30.63

1.33 ± 0.37

7.33 ± 0.88

Interaction

137

 

Milk

 

0

0.19 ± 0.009

0.09 ± 1.01

0.04 ± 0.009

1.20 ± 0.09

Acids

642

9.72

24

0.19 ± 0.009

4.72 ± 0.55

0.04 ± 0.009

2.41 ± 0.18

Time

685

 

72

0.19 ± 0.009

8.98 ± 1.48

0.04 ± 0.009

168.2 ± 11.29

Interaction

618

 
  1. Nd no data
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