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Table 2 Summary of the assays described in the manuscript

From: Methods available to assess therapeutic potential of fibrinolytic enzymes of microbial origin: a review

In vitro assays Characteristics
 Anticoagulant and thrombolytic activity • Tests the anticoagulant and thrombolytic ability of a test compound • Simpler assay
 CloFAL • Periodic quantitative measurement (turbidity) of clot lysis • Analytically sensitive to the components of coagulation and fibrinolytic systems and to physiological changes in haemostasis • Inability to detect mild deficiency in factor VIII
 Euglobulin clot lysis assay • Measures fibrinolysis within the euglobulin fraction • rapid and sensitive method
 Anti-platelet aggregation activity • Tests the inhibition or reduction of platelet aggregation in vitro • Simplicity and ease of use • Does not reflect the platelet function in vivo at all times • Variation in the result obtained due to sample ageing • Interference of other substances with the result • Loss of platelets during PRP preparation
 TEG • Measures the viscoelastic properties of the clot • Sensitive to the changes in the fibrin polymerisation and platelet count • Identifies the unpredictable changes in coagulability during sepsis and disseminated intravascular coagulation • High cost of the equipment
In vivo assays Characteristics
d-Dimer test • Monitors fibrinolysis with the help of cross-linked d-dimer domains • Helps to assess the duration of anticoagulation therapy • Varies with respect to the types of d-dimer unit • Not applicable for fibrinogenolysis
 Ferric chloride-induced thrombosis model • Topical application of ferric chloride leading to vascular wall injury • thrombus formation closest to that in human pathological condition • Sensitive to various thrombin inhibitors • Must be combined with other assays to diagnose/treat DVT
 Carrageenan-induced thrombosis model • Formation of wine coloured thrombus on injecting carrageenan • Simplicity and reproducibility
 Rat groin flap model • Vessel wall stimulating using bipolar stimulating electrode • LDPM employed to monitor blood flow • Short length of the pedicle serves as drawback
 Phytochemical injury • Photo excitation oxidative damage of the vessel wall • Increased phytochemical efficacy • Lower systemic toxicity
 Laser-induced injury • Thrombosis is induced through heat damage • Complete denudation of endothelium