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Table 2 Summary of the assays described in the manuscript

From: Methods available to assess therapeutic potential of fibrinolytic enzymes of microbial origin: a review

In vitro assays Characteristics
 Anticoagulant and thrombolytic activity • Tests the anticoagulant and thrombolytic ability of a test compound
• Simpler assay
 CloFAL • Periodic quantitative measurement (turbidity) of clot lysis
• Analytically sensitive to the components of coagulation and fibrinolytic systems and to physiological changes in haemostasis
• Inability to detect mild deficiency in factor VIII
 Euglobulin clot lysis assay • Measures fibrinolysis within the euglobulin fraction
• rapid and sensitive method
 Anti-platelet aggregation activity • Tests the inhibition or reduction of platelet aggregation in vitro
• Simplicity and ease of use
• Does not reflect the platelet function in vivo at all times
• Variation in the result obtained due to sample ageing
• Interference of other substances with the result
• Loss of platelets during PRP preparation
 TEG • Measures the viscoelastic properties of the clot
• Sensitive to the changes in the fibrin polymerisation and platelet count
• Identifies the unpredictable changes in coagulability during sepsis and disseminated intravascular coagulation
• High cost of the equipment
In vivo assays Characteristics
d-Dimer test • Monitors fibrinolysis with the help of cross-linked d-dimer domains
• Helps to assess the duration of anticoagulation therapy
• Varies with respect to the types of d-dimer unit
• Not applicable for fibrinogenolysis
 Ferric chloride-induced thrombosis model • Topical application of ferric chloride leading to vascular wall injury
• thrombus formation closest to that in human pathological condition
• Sensitive to various thrombin inhibitors
• Must be combined with other assays to diagnose/treat DVT
 Carrageenan-induced thrombosis model • Formation of wine coloured thrombus on injecting carrageenan
• Simplicity and reproducibility
 Rat groin flap model • Vessel wall stimulating using bipolar stimulating electrode
• LDPM employed to monitor blood flow
• Short length of the pedicle serves as drawback
 Phytochemical injury • Photo excitation oxidative damage of the vessel wall
• Increased phytochemical efficacy
• Lower systemic toxicity
 Laser-induced injury • Thrombosis is induced through heat damage
• Complete denudation of endothelium