Table 2 Accuracy of quantifying mephedrone in plasma using standard addition method
Matrix | Calibration curve | X value when Y = 0 | Calculated drug concentration (ng/ml) | % Recovery |
|---|---|---|---|---|
Solvent | Y = 17,282 X + 124,380 | 7.20 ng/ml | ||
Plasma with 0 ng/ml mephedrone | Y = 39,587 X + 1,212,540 | 30.63 ng/ml | ||
Plasma with 10 ng/ml mephedrone | Y = 35,177 X + 1,752,490 | 49.82 ng/ml | 49.82–30.63 = 19.19 ng/ml | 19.19/10 = 191.9% |
Plasma with 33 ng/ml mephedrone | Y = 36,238 X + 2,374,220 | 65.52 ng/ml | 65.52–30.63 = 34.89 ng/ml | 34.89/33 = 105.7% |
Plasma with 100 ng/ml mephedrone | Y = 37,547 X + 5,532,760 | 147.36 ng/ml | 147.37–30.63 = 116.73 ng/ml | 116.73/100 = 116.7% |