Rapid and selective simultaneous quantitative analysis of modified nucleosides using multi-column liquid chromatography-tandem mass spectrometry

Jinno, Daisuke; Kanemitsu, Yoshitomi; Saitoh, Kazuki; Nankumo, Shinnosuke; Tsukamoto, Hiroki; Matsumoto, Yotaro; Abe, Takaaki; Tomioka, Yoshihisa

doi:10.1186/s40543-017-0110-4

Journal of Analytical Science and Technology

Table 4 Accuracy and precision of nucleosides determination

From: Rapid and selective simultaneous quantitative analysis of modified nucleosides using multi-column liquid chromatography-tandem mass spectrometry

Compound	Nominal conc.	Accuracy (%, n = 5)			Precision (CV %, n = 5)
	(ng mL^-1)	Day 1	Day 2	Day 3	Day 1	Day 2	Day 3
A	1.00	80.89	115.1	90.92	8.695	18.96	18.77
	2.00	99.87	100.4	101.6	11.39	14.22	13.4
	100	103.4	106.4	98.96	9.693	13.86	4.715
	200	94.53	103.4	87.53	7.254	4.434	9.463
m¹A	1.25	112	99.07	92.01	17.5	17.31	18.39
	2.50	98.75	104.2	89.22	14.8	13.63	6.688
	250	104.7	96.29	107.4	2.781	14.01	5.248
	500	105.9	99.13	100.7	5.26	12.35	3.37
I	2.50	80.03	101.2	89.46	17.17	11.15	3.536
	5.00	106	95.87	104.1	14.76	11.5	11.21
	250	98.16	105.8	101.3	5.065	12.14	4.513
	500	85.72	89.05	98.19	4.975	4.251	9.22
G	2.50	96.91	82.1	91.72	11.76	19.92	13.07
	5.00	87.73	104	104.8	13.68	8.85	13.57
	250	107.2	113.4	107.5	5.877	6.036	3.147
	500	98.92	109.1	101.5	6.147	4.587	4.092
m²G	10.0	112.5	104.1	106.3	14.64	14.14	6.845
	20.0	91.89	102.6	104.1	7.714	13	7.213
	1000	108.8	107.1	105.8	4.05	4.05	3.614
	2000	101.5	100.1	97.28	8.088	8.087	4.625
m⁷G	12.5	101	105.7	113.6	19.92	9.69	16.52
	25.0	96.96	93.67	106.4	13.45	8.244	13.57
	250	101.4	112.2	96.06	5.524	4.694	7.28
	500	102.9	103.3	99.69	7.926	6.845	4.815
m² ₂G	1.00	119.6	119.1	115.9	13.68	14.68	7.302
	2.00	86.04	93.41	86.09	9.516	11.8	12.52
	1000	109	103.7	112.2	6.465	5.908	10.27
	2000	99.07	96.76	101.6	3.59	2.827	3.27
C	2.50	82.88	106.6	117.5	18.67	17.03	7.551
	5.00	114.7	94.29	107.9	14.71	8.268	14.39
	250	102.4	109.3	97.02	5.971	4.065	12.05
	500	96.1	102.9	95.4	1.073	2.961	9.001
m³C	1.39	84.14	111.2	106.4	19.35	13.05	8.499
	3.48	104.8	113	96.75	14.17	13.6	6.68
	348	105.9	104	100.4	1.999	5.678	2.158
	696	92.01	95.75	94.3	3.692	12.98	4.793
m⁵C	0.50	81.32	106.6	117	15.81	7.609	2.149
	1.00	94.27	110.2	101.8	8.507	9.095	1.588
	250	93.69	95.94	93.64	3.874	5.248	6.573
	500	89.01	85.05	89.83	4.385	3.218	3.484
Cm	1.00	86.72	119	93.69	13.98	14.89	17.82
	2.00	92.96	86.7	99.21	14.84	13.67	9.533
	200	103.1	113.6	111.1	5.603	7.017	3.349
	400	95.17	106.6	100.6	3.041	4.291	6.287
s²C	4.39	102.8	119.5	107	18.05	13.98	7.756
	8.77	98.38	91.49	95.23	12.52	10.3	13.62
	87.7	106.7	109.8	111.6	9.275	4.069	5.446
	175	108.2	90.96	99.43	10.06	9.576	3.505
U	12.5	98.98	106.7	103.2	18.14	7.711	1.731
	25.0	102.3	99.52	94.26	12.13	7.518	10.73
	250	102.7	98.59	105.6	9.811	9.06	2.607
	500	108.8	105.6	103.7	10.47	6.208	5.767
m⁵U	50.0	106.7	109.8	117.6	15.21	3.571	6.369
	100	91.63	89.08	94.54	10.2	4.907	11.57
	1000	111.1	102.4	109.5	5.757	5.617	4.958
	2000	104	110.3	112.1	8.855	5.899	2.927
Ψ	5.00	97.95	108.5	99.8	17.19	9.301	17.63
	12.5	98.89	95.34	99.66	12.16	7.573	9.511
	250	108.8	99.72	94.49	6.829	6.079	5.563
	500	96.65	95.83	101.5	7.376	4.857	2.329

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