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Table 4 Accuracy and precision of nucleosides determination

From: Rapid and selective simultaneous quantitative analysis of modified nucleosides using multi-column liquid chromatography-tandem mass spectrometry

Compound Nominal conc. Accuracy (%, n = 5) Precision (CV %, n = 5)
  (ng mL-1) Day 1 Day 2 Day 3 Day 1 Day 2 Day 3
A 1.00 80.89 115.1 90.92 8.695 18.96 18.77
  2.00 99.87 100.4 101.6 11.39 14.22 13.4
  100 103.4 106.4 98.96 9.693 13.86 4.715
  200 94.53 103.4 87.53 7.254 4.434 9.463
m1A 1.25 112 99.07 92.01 17.5 17.31 18.39
  2.50 98.75 104.2 89.22 14.8 13.63 6.688
  250 104.7 96.29 107.4 2.781 14.01 5.248
  500 105.9 99.13 100.7 5.26 12.35 3.37
I 2.50 80.03 101.2 89.46 17.17 11.15 3.536
  5.00 106 95.87 104.1 14.76 11.5 11.21
  250 98.16 105.8 101.3 5.065 12.14 4.513
  500 85.72 89.05 98.19 4.975 4.251 9.22
G 2.50 96.91 82.1 91.72 11.76 19.92 13.07
  5.00 87.73 104 104.8 13.68 8.85 13.57
  250 107.2 113.4 107.5 5.877 6.036 3.147
  500 98.92 109.1 101.5 6.147 4.587 4.092
m2G 10.0 112.5 104.1 106.3 14.64 14.14 6.845
  20.0 91.89 102.6 104.1 7.714 13 7.213
  1000 108.8 107.1 105.8 4.05 4.05 3.614
  2000 101.5 100.1 97.28 8.088 8.087 4.625
m7G 12.5 101 105.7 113.6 19.92 9.69 16.52
  25.0 96.96 93.67 106.4 13.45 8.244 13.57
  250 101.4 112.2 96.06 5.524 4.694 7.28
  500 102.9 103.3 99.69 7.926 6.845 4.815
m2 2G 1.00 119.6 119.1 115.9 13.68 14.68 7.302
  2.00 86.04 93.41 86.09 9.516 11.8 12.52
  1000 109 103.7 112.2 6.465 5.908 10.27
  2000 99.07 96.76 101.6 3.59 2.827 3.27
C 2.50 82.88 106.6 117.5 18.67 17.03 7.551
  5.00 114.7 94.29 107.9 14.71 8.268 14.39
  250 102.4 109.3 97.02 5.971 4.065 12.05
  500 96.1 102.9 95.4 1.073 2.961 9.001
m3C 1.39 84.14 111.2 106.4 19.35 13.05 8.499
  3.48 104.8 113 96.75 14.17 13.6 6.68
  348 105.9 104 100.4 1.999 5.678 2.158
  696 92.01 95.75 94.3 3.692 12.98 4.793
m5C 0.50 81.32 106.6 117 15.81 7.609 2.149
  1.00 94.27 110.2 101.8 8.507 9.095 1.588
  250 93.69 95.94 93.64 3.874 5.248 6.573
  500 89.01 85.05 89.83 4.385 3.218 3.484
Cm 1.00 86.72 119 93.69 13.98 14.89 17.82
  2.00 92.96 86.7 99.21 14.84 13.67 9.533
  200 103.1 113.6 111.1 5.603 7.017 3.349
  400 95.17 106.6 100.6 3.041 4.291 6.287
s2C 4.39 102.8 119.5 107 18.05 13.98 7.756
  8.77 98.38 91.49 95.23 12.52 10.3 13.62
  87.7 106.7 109.8 111.6 9.275 4.069 5.446
  175 108.2 90.96 99.43 10.06 9.576 3.505
U 12.5 98.98 106.7 103.2 18.14 7.711 1.731
  25.0 102.3 99.52 94.26 12.13 7.518 10.73
  250 102.7 98.59 105.6 9.811 9.06 2.607
  500 108.8 105.6 103.7 10.47 6.208 5.767
m5U 50.0 106.7 109.8 117.6 15.21 3.571 6.369
  100 91.63 89.08 94.54 10.2 4.907 11.57
  1000 111.1 102.4 109.5 5.757 5.617 4.958
  2000 104 110.3 112.1 8.855 5.899 2.927
Ψ 5.00 97.95 108.5 99.8 17.19 9.301 17.63
  12.5 98.89 95.34 99.66 12.16 7.573 9.511
  250 108.8 99.72 94.49 6.829 6.079 5.563
  500 96.65 95.83 101.5 7.376 4.857 2.329