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Table 3 LC-MS/MS and calibration curve parameters for nucleosides

From: Rapid and selective simultaneous quantitative analysis of modified nucleosides using multi-column liquid chromatography-tandem mass spectrometry

Compound tR Transition Calibration range Correlation coefficient
  (min) (m/z) (ng mL−1)
A 5.13 268.1 → 136.1 1.00–200 0.9993
m1A 3.01 282.1 → 150.1 1.25–500 0.9983
I 3.63 269.1 → 137.1 2.50–500 0.9987
G 3.76 284.1 → 152.1 2.50–500 0.9989
m2G 3.48 298.1 → 166.1 10.0–2000 0.9998
m7G 4.71 298.1 → 166.1 12.5–500 0.9983
m2 2G 5.47 312.1 → 180.1 1.00–2000 0.9999
C 2.27 244.1 → 112.1 2.50–500 0.9994
m3C 3.62 258.1 → 126.1 1.39–696a 0.9999
m5C 2.33 258.1 → 126.1 0.50–500 0.9999
Cm 3.80 258.1 → 112.1 1.00–400 0.9984
s2C 3.28 260.1 → 128.1 4.39–175b 0.9977
U 2.74 245.1 → 113.1 12.5–500 0.9979
m5U 3.99 259.1 → 127.1 50–2000 0.9973
Ψ 1.56 245.1 → 209.0 5.00–500 0.9994
f2dA (IS) 6.24 270.2 → 154.2
  1. aNucleoside test mix contained 100 μg mL−1 of 3-methylcytidine methosulfate, and 69.6 μg mL−1 of m3C
  2. bNucleoside test mix contained 10 μg mL−1 of 2-thiocytidine dihydrate and 8.77 μg mL−1 of s2C