Table 3 LC-MS/MS and calibration curve parameters for nucleosides

A

5.13

268.1 → 136.1

1.00–200

0.9993

m¹A

3.01

282.1 → 150.1

1.25–500

0.9983

I

3.63

269.1 → 137.1

2.50–500

0.9987

G

3.76

284.1 → 152.1

2.50–500

0.9989

m²G

3.48

298.1 → 166.1

10.0–2000

0.9998

m⁷G

4.71

298.1 → 166.1

12.5–500

0.9983

m² ₂G

5.47

312.1 → 180.1

1.00–2000

0.9999

C

2.27

244.1 → 112.1

2.50–500

0.9994

m³C

3.62

258.1 → 126.1

1.39–696^a

0.9999

m⁵C

2.33

258.1 → 126.1

0.50–500

0.9999

Cm

3.80

258.1 → 112.1

1.00–400

0.9984

s²C

3.28

260.1 → 128.1

4.39–175^b

0.9977

U

2.74

245.1 → 113.1

12.5–500

0.9979

m⁵U

3.99

259.1 → 127.1

50–2000

0.9973

Ψ

1.56

245.1 → 209.0

5.00–500

0.9994

f²dA (IS)

6.24

270.2 → 154.2

–

–