Method development, validation, and stability studies of teneligliptin by RP-HPLC and identification of degradation products by UPLC tandem mass spectroscopy

Journal of Analytical Science and Technology

Table 7 HPLC data of Degradation studies

Stress condition	Stress parameters used	Time (hours)	Drug peak		Degraded product peaks
			Rt^a	Peak intensity	Degraded product peaks
					Peak 1		Peak 2
					Rt^a	Intensity	Rt^a	Intensity
Acid	0.1 N HCl	48	5.035	594.73	–	–	–	–
Base	0.1 N NaOH	48	5.177	364.46	2.966	312.129	–	–
Oxidative	0.3%H₂O₂	48	5.299	1899.8	2.519	846.61	2.979	857.45
Light	UV Light	48	5.150	457.29	–	–	–	–
Temperature	60 °C	48	5.140	600.41	3.033	46.65	–	–