An effective and sensitive stability-indicating chromatographic approach based on HPLC for silodosin assay

Journal of Analytical Science and Technology

Table 3 Validation results obtained from spectrofluorimetry for the quantitative determination of SIL

Parameters	Spectrofluorimetry
Wavelength (nm)	λex = 330, λem = 460
Linearity range^a (μM)	0.2–36.4
Slope^b	26.993
Intercept^b	4.4526
Correlation coefficient (R ²)	0.9975
LOD (nM)	7.70
LOQ (nM)	26.80
Repeatability of peak intensity (RSD %)^c	0.48
Repeatability of peak intensity (RSD %)^d	0.85

^aAverage of three determinations
^b I _FL (a.u.) = m C _SL (μM) + b where C is the concentration and I _FL is the fluorescence intensities
^cIntra-day measurements—each value is the mean of five experiments
^dInter-day measurements—each value is the mean of five experiments