Table 6 Stability studies of analytes in human plasma
Analyte | Stability check | Samples | Averagea nominal conc. | Averagea observed conc. | % CV | % Stability |
|---|---|---|---|---|---|---|
(ng/ml) | (ng/ml) | |||||
Lamivudine | Bench top (7.0 h) | LQC | 27.53 | 27.76 | 2.34 | 100.84 |
HQC | 1146.90 | 1161.87 | 1.77 | 102.31 | ||
Freeze thaw (4 cycles) | LQC | 27.53 | 28.16 | 2.52 | 102.26 | |
HQC | 1146.90 | 1155.10 | 2.97 | 101.71 | ||
In-auto-sampler (22 h) | LQC | 27.53 | 27.95 | 4.44 | 101.50 | |
HQC | 1146.90 | 1159.58 | 1.63 | 102.11 | ||
Wet extract (7 h) | LQC | 27.53 | 27.72 | 2.76 | 100.69 | |
HQC | 1146.90 | 1150.12 | 1.65 | 101.27 | ||
Zidovudine | Bench top (7.0 h) | LQC | 29.84 | 28.43 | 3.38 | 97.79 |
HQC | 1243.43 | 1212.32 | 2.06 | 100.06 | ||
Freeze thaw (4 cycles) | LQC | 29.84 | 30.02 | 1.50 | 103.24 | |
HQC | 1243.43 | 1206.04 | 1.71 | 99.54 | ||
In-auto-sampler (22 h) | LQC | 29.84 | 28.97 | 5.08 | 99.66 | |
HQC | 1243.43 | 1212.50 | 1.43 | 100.07 | ||
Wet extract (7 h) | LQC | 29.84 | 28.32 | 5.96 | 97.40 | |
HQC | 1243.43 | 1210.84 | 2.51 | 99.94 | ||
Nevirapine | Bench top (7.0 h) | LQC | 44.29 | 45.33 | 2.18 | 101.73 |
HQC | 1845.48 | 1821.13 | 1.08 | 102.24 | ||
Freeze thaw (4 cycles) | LQC | 44.29 | 46.25 | 2.82 | 103.80 | |
HQC | 1845.48 | 1816.07 | 1.34 | 101.96 | ||
In-auto-sampler (22 h) | LQC | 44.29 | 45.38 | 3.30 | 101.84 | |
HQC | 1845.48 | 1829.70 | 1.44 | 102.73 | ||
Wet extract (7 h) | LQC | 44.29 | 44.48 | 1.54 | 99.81 | |
HQC | 1845.48 | 1795.11 | 1.76 | 100.78 |