GC-FID determination of nucleobases guanine, adenine, cytosine, and thymine from DNA by precolumn derivatization with isobutyl chloroformate

Journal of Analytical Science and Technology

Table 2 Analysis of acid-hydrolyzed DNA of human blood and cotton, rice, and jasmine leaves for T, C, A, and G concentration

Purine and pyrimidine bases	Blood sample 1 (μmol L⁻¹)	Blood sample 2 (μmol L⁻¹)	Blood sample 3 (μmol L⁻¹)	Blood sample 4 (μmol L⁻¹)	Blood sample 5(μmol L⁻¹)	Cotton (nmol g⁻¹)	Rice (nmol g⁻¹)	Jasmine (nmol g⁻¹)
T	1.673 (0.81)	0.994 (1.83)	2.600 (2.55)	2.625 (0.76)	1.895 (4.13)	0.825 (5.00)	0.521 (4.85)	0.952 (4.00)
	1.672^a (2.51)					0.818^a (4.50)	0.524^a (3.54)	0.950^a (2.44)
C	1.563 (2.65)	1.040 (3.45)	1.740 (0.82)	1.352 (4.51)	1.475 (5.00)	2.915 (4.40)	1.038 (2.98)	0.490 (3.96)
	1.564^a (0.50)					2.913^a (1.00)	1.038^a (1.78)	0.478^a (4.55)
A	1.716 (0.77)	1.012 (1.93)	2.609 (5.00)	2.655 (2.89)	1.961 (4.99)	0.835 (0.88)	0.559 (3.00)	0.899 (1.11)
	1.718^a (1.77)					0.876^a (2.94)	0.561^a (1.95)	0.895^a (4.11)
G	1.585 (2.22)	0.988 (3.33)	1.759 (5.66)	1.408 (4.99)	1.591 (2.99)	2.739 (4.00)	1.108 (1.66)	0.480 (4.55)
	1.561^a (0.99)					2.739^a (1.77)	1.105^a (3.98)	0.468^a (0.50)