Table 1 Results of quantitation of purine and pyrimidine bases using isobutyl chloroformate derivatizing reagent
Purine and pyrimidine bases | Limits of detection (LOD) (μmol L−1) | Limits of quantitation (LOQ) (μmol L−1) | Calibration range (μmol L−1) | Coefficient of determination (r 2) |
|---|---|---|---|---|
Thymine (T) | 0.09 | 0.27 | 0.5–50 | 0.991 |
Uracil (U) | 0.09 | 0.27 | 0.5–50 | 0.990 |
Adenine (A) | 0.08 | 0.24 | 0.5–50 | 0.992 |
Cytosine (C) | 0.10 | 0.3 | 0.5–50 | 0.993 |
Guanine (G) | 0.10 | 0.3 | 0.5–50 | 0.992 |