From: Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
Copies (MERS-CoV IVT)/reaction | |||||||
---|---|---|---|---|---|---|---|
102 | 101.5 | 101 | 100.5 | 100 | 10−0.5 | 10−1 | |
Aptamer (25 μM/reaction) | 24/24 | 24/24 | 24/24 | 19/24 | 11/24 | 2/24 | 1/24 |
100 % | 100 % | 100 % | 79.17 % | 45.83 % | 8.33 % | 4.17 % | |
Control (without aptamer) | 24/24 | 24/24 | 22/24 | 13/24 | 5/24 | 2/24 | 0/24 |
100 % | 100 % | 91.67 % | 54.17 % | 20.83 % | 8.33 % | 0 % |