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Table 1 Hit rate of 25 μM aptamer and without aptamer in real-time RT-PCR MERS-CoV assay. Half-logarithmic serial dilutions of MERS-CoV RNA, ranging from 10−1 to 102 copies per reaction were analyzed. The ratio of positive signals to all signals, followed by the data in percentage is given below

From: Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance

 

Copies (MERS-CoV IVT)/reaction

 

102

101.5

101

100.5

100

10−0.5

10−1

Aptamer (25 μM/reaction)

24/24

24/24

24/24

19/24

11/24

2/24

1/24

100 %

100 %

100 %

79.17 %

45.83 %

8.33 %

4.17 %

Control (without aptamer)

24/24

24/24

22/24

13/24

5/24

2/24

0/24

100 %

100 %

91.67 %

54.17 %

20.83 %

8.33 %

0 %