Fig. 1
From: Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
Relation between aptamer concentration and cycle threshold (C t ) at 31 and 55 °C. Aptamer concentrations of 0, 12.5, 25, and 50 μM per reaction (a) and 0, 50, 100, and 200 μM per reaction (b) were tested in a one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using 1000 RNA copies per reaction. RT-step was carried out for 20 min in parallel at 31 °C (circles) and 55 °C (dots), respectively. Results of 200 μM aptamer concentration at 31 °C did not lead to a positive signal and therefore are not shown