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Fig. 2 | Journal of Analytical Science and Technology

Fig. 2

From: Middle-down electron capture dissociation and electron transfer dissociation for histone analysis

Fig. 2

Example of a theoretical tryptic digestion of the N-terminus of H3.1 histone leading to peptides generally smaller than six amino acids that would not be amenable to LC-MS/MS in a typical bottom-up approach. Only two peptides (in blue circles) would be detectable. In addition, peptide PGTVALR is shared between histone H3.1 and histone H3.2, which makes it impossible to infer from which protein it originally came from. Middle-down approaches circumvent these problems by looking at larger peptides (in the case of histone H3.1 with 50 amino acids)

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